Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
ternary and binary complexes with no metals, Na+ or K+, ATP (orATP-gammaS), and SP20 protein, sitting drop vapor diffusion method, using 100 mM MES-NH4OH, pH 6.5, 5 mM dithiothreitol, 15-20% (w/v) PEG 4000 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PKI | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | highest activity in the presence of Mg2+ | Mus musculus | |
additional information | not activated by Na+, K+, and Ca2+ | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P05132 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, and gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + SP20 protein | i.e. TTYADFIASGRTGRRASIHD | Mus musculus | ADP + phosphorylated SP20 protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKAc | catalytic subunit of cAMP-dependent protein kinase | Mus musculus |