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Literature summary for 2.7.11.11 extracted from
- Probes of the mitochondrial cAMP-dependent protein kinase (2013), Biochim. Biophys. Acta, 1834, 1359-1363.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | oligomycin, antimycin A, rotenone, CCCP, sodium azide, or Ca2+ activate calpain which activates the cAMP-dependent protein kinase | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
N-(8-([4-[3-(ethoxycarbonyl)-6,8,8-trimethyl-2-oxo-7,8-dihydro-2H-pyrano[3,2-g]quinolin-9(6H)-yl]butanoyl]amino)octanoyl)-GRTGRRFSYP-amide | pH and temperature not specified in the publication | Bos taurus |  |
| 0.0022 | - |
N-(8-([[7-(diethylamino)-2-oxo-2H-chromen-3-yl]carbonyl]amino)octanoyl)-GRTGRRFSYP-amide | pH and temperature not specified in the publication | Bos taurus | |
| 0.0062 | - |
N-(8-[[(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-10-yl)carbonyl]amino]octanoyl)-GRTGRRFSYP-amide | pH and temperature not specified in the publication | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | associated with the inner membrane/matrix | Bos taurus | 5739 | - |
| additional information | the enzyme is anchored to a variety of intracellular locations via interaction with A-kinase anchoring proteins | Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+ activates calpain which activates the cAMP-dependent protein kinase | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the cAMP-dependent protein kinase is a serine/threonine kinase that exists as an inactive tetrameric holoenzyme consisting of two regulatory subunits and two catalytic subunits. It is activated by digestion of the regulatory subunits through calpain releasing the active catalytic ssubunits | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-(8-([4-[3-(ethoxycarbonyl)-6,8,8-trimethyl-2-oxo-7,8-dihydro-2H-pyrano[3,2-g]quinolin-9(6H)-yl]butanoyl]amino)octanoyl)-GRTGRRFSYP-amide | - |
Bos taurus | ADP + N-(8-([4-[3-(ethoxycarbonyl)-6,8,8-trimethyl-2-oxo-7,8-dihydro-2H-pyrano[3,2-g]quinolin-9(6H)-yl]butanoyl]amino)octanoyl)-GRTGRRFpSYP-amide | - |
? | |
| ATP + N-(8-([[7-(diethylamino)-2-oxo-2H-chromen-3-yl]carbonyl]amino)octanoyl)-GRTGRRFSYP-amide | - |
Bos taurus | ADP + N-(8-([[7-(diethylamino)-2-oxo-2H-chromen-3-yl]carbonyl]amino)octanoyl)-GRTGRRFpSYP-amide | - |
? | |
| ATP + N-(8-[[(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-10-yl)carbonyl]amino]octanoyl)-GRTGRRFSYP-amide | - |
Bos taurus | ADP + N-(8-[[(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-10-yl)carbonyl]amino]octanoyl)-GRTGRRFpSYP-amide | - |
? | |
| additional information | development of kinase activity sensor fluorogenic substrates, upon phosphorylation the peptide interacts with a phosphoserine-binding protein 14-3-3 domain, displacing the quencher, and resulting in a burst of fluorescence, overview | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | beta2, the cAMP-dependent protein kinase is a serine/threonine kinase that exists as an inactive tetrameric holoenzyme consisting of two regulatory subunits and two catalytic subunits. It is activated by digestion of the regulatory subunits through calpain releasing the active catalytic ssubunits | Bos taurus |
| tetramer | alpha2beta2, the cAMP-dependent protein kinase is a serine/threonine kinase that exists as an inactive tetrameric holoenzyme consisting of two regulatory subunits and two catalytic subunits. It is activated by digestion of the regulatory subunits through calpain | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PKA | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | PKA activity at the mitochondria is associated with the regulation of apoptosis, mitochondrial respiration, and ATP synthesis. Mitochondrial cAMP-dependent protein kinase is regulted by the protease calpain. Upon exposure of bovine heart mitochondria to digitonin, Ca2+, and a variety of electron transport chain inhibitors, the regulatory subunits of the PKA holoenzyme are digested by calpain, releasing active catalytic subunits. The proteolysis can be attenuated by calpain inhibitor I | Bos taurus |
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