Cloned (Comment) | Organism |
---|---|
gene RKA1, regulatory subunit, located in the F chromosome, expression of His6-tagged R subunit in Escherichia coli strain BL21(DE3) | Yarrowia lipolytica |
gene TPK1 | Yarrowia lipolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Yarrowia lipolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42800 | - |
1 * 42800, about, regulatory subunit, + 1 x 39000, catalytic subunit, sequence calculation | Yarrowia lipolytica |
48000 | - |
1 * 48000, isolated recombinant His-tagged regulatory subunit, SDS-PAGE | Yarrowia lipolytica |
90000 | - |
holoenzyme, gel filtration | Yarrowia lipolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | C3W7W0 | catalytic subunit, gene TPK1; strains PO1a and AC11, genes TPK1 and RKA1 encoding catalytic and regulatory subunits | - |
Yarrowia lipolytica | F2XG10 | regulatory subunit, gene RKA1; strains PO1a and AC11, genes TPK1 and RKA1 encoding catalytic and regulatory subunits | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the catalytic subunits phosphorylate the regulatory subunits | Yarrowia lipolytica |
Purification (Comment) | Organism |
---|---|
native catalytic subunit from by anion exchange and cAMP affinity chromatography | Yarrowia lipolytica |
recombinant His6-tagged R subunit from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography | Yarrowia lipolytica |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 42800, about, regulatory subunit, + 1 x 39000, catalytic subunit, sequence calculation | Yarrowia lipolytica |
monomer | 1 * 48000, isolated recombinant His-tagged regulatory subunit, SDS-PAGE | Yarrowia lipolytica |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Yarrowia lipolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Yarrowia lipolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Yarrowia lipolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Yarrowia lipolytica | regulatory subunit, sequence calculation | - |
5.18 |