Protein Variants | Comment | Organism |
---|---|---|
K336A/H338A | i.e. Tpk1K336A/H338A, site-directed mutagenesis, a catalytically inactive PKA variant, that exhibits a stable binding to the substrate | Saccharomyces cerevisiae |
additional information | TPK1 overexpression resulted in a severe growth defect | Saccharomyces cerevisiae |
R324A | i.e. Tpk1R324A, site-directed mutagenesis, a catalytically inactive PKA variant, that exhibits a stable binding to the substrate with increased affinity through a conformational change | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | PKA associates with substrates with a relatively low affinity | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Dot6 | Saccharomyces cerevisiae | a protein implicated in telomere function | ADP + phosphorylated Dot6 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
several strains, overview | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | conserved residues K336 and H338 are important for catalytic activity, R324 is important in substrate binding | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Dot6 | a protein implicated in telomere function | Saccharomyces cerevisiae | ADP + phosphorylated Dot6 | - |
? | |
ATP + Dot6 | a protein implicated in telomere function, substrate of Tpk1 | Saccharomyces cerevisiae | ADP + phosphorylated Dot6 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Saccharomyces cerevisiae |
Tpk1 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |