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Literature summary for 2.7.11.11 extracted from

  • Yang, J.; Ten Eyck, L.F.; Xuong, N.H.; Taylor, S.S.
    Crystal structure of a cAMP-dependent protein kinase mutant at 1.26A: new insights into the catalytic mechanism (2004), J. Mol. Biol., 336, 473-487.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant Y204A in Escherichia coli Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and mutant PKA catalytic subunits, 0.002 ml 5 mg/ml protein in 50 mM bicine, pH 8.0, 200 mM ammonium acetate, 2 mM DTT, MgCl2, ATP, and IP20, is mixed with 0.001 ml well solution containing 8-12% v/v 2-methyl-2,4-pentanediol and 10 mM DTT, addition of 7% v/v methanol before sealing, X-ray diffraction structure determination and analysis at 1.26 A resolution, modeling Mus musculus

Protein Variants

Protein Variants Comment Organism
Y204A site-directed mutagenesis, mutation in the catalytic subunit, reduced catalytic efficiency compared to the wild-type enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
IP20 inhibitory peptide TTYADFIASGRTGRRN, residues 5-24 of inhibitor PKI, inhibits the ATPase function of the enzyme Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P05132
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant Y204A from Escherichia coli by ion exchange chromatography and gel filtration Mus musculus

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate catalytic mechanism, active site structure, Tyr204 is required and involved in the hydrophobic network, substrate-induced interaction of Tyr204 and Asp166, overview Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Kemptide LRRASLG peptide substrate Mus musculus ADP + Kemptide phosphate
-
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Synonyms

Synonyms Comment Organism
PKA
-
Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus