Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant Y204A in Escherichia coli | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and mutant PKA catalytic subunits, 0.002 ml 5 mg/ml protein in 50 mM bicine, pH 8.0, 200 mM ammonium acetate, 2 mM DTT, MgCl2, ATP, and IP20, is mixed with 0.001 ml well solution containing 8-12% v/v 2-methyl-2,4-pentanediol and 10 mM DTT, addition of 7% v/v methanol before sealing, X-ray diffraction structure determination and analysis at 1.26 A resolution, modeling | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
Y204A | site-directed mutagenesis, mutation in the catalytic subunit, reduced catalytic efficiency compared to the wild-type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
IP20 | inhibitory peptide TTYADFIASGRTGRRN, residues 5-24 of inhibitor PKI, inhibits the ATPase function of the enzyme | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P05132 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant Y204A from Escherichia coli by ion exchange chromatography and gel filtration | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | catalytic mechanism, active site structure, Tyr204 is required and involved in the hydrophobic network, substrate-induced interaction of Tyr204 and Asp166, overview | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Kemptide | LRRASLG peptide substrate | Mus musculus | ADP + Kemptide phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |