Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | each monomer of the tetrameric enzyme contains 2 bindig sites A and B for cAMP, binding involves Arg230 of domain A and Arg359 in domain B of regulatory subunit RIIbeta, cAMP binding releases the catalytic subunits | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged catalytic subunit C, and His-tagged wild-type and mutant regulatory subunits RIIbeta | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a deletion mutant of regulatory subunit RIIbeta lacking the complete domain B | Rattus norvegicus |
R230K | site-directed mutagenesis, mutation of an A domain residue of regulatory subunit RIIbeta, the mutant possesses one high-affinity cAMP binding site and one low active binding site resulting in reduced overall cAMP binding | Rattus norvegicus |
R359K | site-directed mutagenesis, mutation of a B domain residue of regulatory subunit RIIbeta, the mutant possesses one high-affinity cAMP binding site and one low active binding site resulting in reduced overall cAMP binding | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics for cAMP binding to wild-type and mutant regulatory subunits RIIbeta, overview | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged catalytic subunit C, and His-tagged wild-type and mutant regulatory subunits RIIbeta, by nickel affinity chromatography and dialysis | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
reversible unfolding and folding in 8.5 M urea of recombinant His-tagged wild-type and mutant regulatory subunits RIIbeta, overview | Rattus norvegicus |
Subunits | Comment | Organism |
---|---|---|
More | holoenzyme structure | Rattus norvegicus |
tetramer | 2 regulatory subunits in a dimer bound to 2 catalytic subunits, the latter are released during activation by cAMP | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
cAPK | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |