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Literature summary for 2.7.11.11 extracted from
- Tandem mass spectrometry identifies proteins phosphorylated by cyclic AMP-dependent protein kinase when sea urchin sperm undergo the acrosome reaction (2005), Dev. Biol., 285, 116-125.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cAMP | dependent on | Strongylocentrotus purpuratus |  |
| additional information | egg jelly layer EJ component fucose sulfate polymer triggers sperm PKA activation | Strongylocentrotus purpuratus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H-85 | unspecific kinase inhibitor, inhibits 23% of the acrosome reaction in sperm at 0.03 mM | Strongylocentrotus purpuratus | |
| H-89 | PKA inhibitor, inhibits 87% of the acrosome reaction in sperm at 0.03 mM | Strongylocentrotus purpuratus | |
| myristoylated PKA peptide inhibitor | 14-22 amide, blocks egg jelly layer induction of PKA | Strongylocentrotus purpuratus | |
| Ni2+ | inhibits sperm protein phosphorylation by the enzyme | Strongylocentrotus purpuratus | |
| nifedipine | inhibits sperm protein phosphorylation by the enzyme | Strongylocentrotus purpuratus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | extracellular Ca2+ is required for enzyme activity in sperm | Strongylocentrotus purpuratus | |
| Mg2+ | - |
Strongylocentrotus purpuratus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + actin | Strongylocentrotus purpuratus | sperm protein substrate | ADP + phosphorylated actin | - |
? | |
| ATP + adenylate kinase 1 | Strongylocentrotus purpuratus | sperm protein substrate | ADP + phosphorylated adenylate kinase 1 | - |
? | |
| ATP + adenylate kinase 5 | Strongylocentrotus purpuratus | sperm protein substrate | ADP + phosphorylated adenylate kinase 5 | - |
? | |
| ATP + creatine kinase | Strongylocentrotus purpuratus | sperm protein substrate | ADP + phosphorylated creatine kinase | - |
? | |
| ATP + EPS8 protein | Strongylocentrotus purpuratus | sperm protein substrate | ADP + EPS8 phosphoprotein | - |
? | |
| ATP + PDE11A protein | Strongylocentrotus purpuratus | sperm protein substrate | ADP + PDE11A phosphoprotein | - |
? | |
| ATP + PDE5A protein | Strongylocentrotus purpuratus | sperm protein substrate | ADP + PDE5A phosphoprotein | - |
? | |
| ATP + ubiquinol-cytochrome c reductase complex core protein 2 | Strongylocentrotus purpuratus | sperm protein substrate | ADP + ubiquinol-cytochrome c reductase complex core phosphoprotein 2 | - |
? | |
| additional information | Strongylocentrotus purpuratus | cAMP concentration and PKA activity are increased and important in exocytotic acrosome reaction taking place in fertilization when sperm contacts the egg jelly layer EJ, EJ component fucose sulfate polymer triggers sperm PKA activation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Strongylocentrotus purpuratus | - |
sea urchin | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| semen | - |
Strongylocentrotus purpuratus | - |
| sperm | - |
Strongylocentrotus purpuratus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + actin | - |
Strongylocentrotus purpuratus | ADP + phosphorylated actin | - |
? | |
| ATP + actin | sperm protein substrate | Strongylocentrotus purpuratus | ADP + phosphorylated actin | - |
? | |
| ATP + adenylate kinase 1 | - |
Strongylocentrotus purpuratus | ADP + phosphorylated adenylate kinase 1 | - |
? | |
| ATP + adenylate kinase 1 | sperm protein substrate | Strongylocentrotus purpuratus | ADP + phosphorylated adenylate kinase 1 | - |
? | |
| ATP + adenylate kinase 5 | - |
Strongylocentrotus purpuratus | ADP + phosphorylated adenylate kinase 5 | - |
? | |
| ATP + adenylate kinase 5 | sperm protein substrate | Strongylocentrotus purpuratus | ADP + phosphorylated adenylate kinase 5 | - |
? | |
| ATP + creatine kinase | - |
Strongylocentrotus purpuratus | ADP + phosphorylated creatine kinase | - |
? | |
| ATP + creatine kinase | sperm protein substrate | Strongylocentrotus purpuratus | ADP + phosphorylated creatine kinase | - |
? | |
| ATP + EPS8 protein | - |
Strongylocentrotus purpuratus | ADP + EPS8 phosphoprotein | - |
? | |
| ATP + EPS8 protein | sperm protein substrate | Strongylocentrotus purpuratus | ADP + EPS8 phosphoprotein | - |
? | |
| ATP + PDE11A protein | - |
Strongylocentrotus purpuratus | ADP + PDE11A phosphoprotein | - |
? | |
| ATP + PDE11A protein | sperm protein substrate | Strongylocentrotus purpuratus | ADP + PDE11A phosphoprotein | - |
? | |
| ATP + PDE5A protein | - |
Strongylocentrotus purpuratus | ADP + PDE5A phosphoprotein | - |
? | |
| ATP + PDE5A protein | sperm protein substrate | Strongylocentrotus purpuratus | ADP + PDE5A phosphoprotein | - |
? | |
| ATP + ubiquinol-cytochrome c reductase complex core protein 2 | - |
Strongylocentrotus purpuratus | ADP + ubiquinol-cytochrome c reductase complex core phosphoprotein 2 | - |
? | |
| ATP + ubiquinol-cytochrome c reductase complex core protein 2 | sperm protein substrate | Strongylocentrotus purpuratus | ADP + ubiquinol-cytochrome c reductase complex core phosphoprotein 2 | - |
? | |
| additional information | cAMP concentration and PKA activity are increased and important in exocytotic acrosome reaction taking place in fertilization when sperm contacts the egg jelly layer EJ, EJ component fucose sulfate polymer triggers sperm PKA activation | Strongylocentrotus purpuratus | ? | - |
? | |
| additional information | sperm protein substrate identification using peptide analysis via tandem mass spectrometry | Strongylocentrotus purpuratus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyclic AMP-dependent protein kinase | - |
Strongylocentrotus purpuratus |
| PKA | - |
Strongylocentrotus purpuratus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
in vivo assay at | Strongylocentrotus purpuratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Strongylocentrotus purpuratus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Strongylocentrotus purpuratus | |
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