Literature summary for 2.7.11.10 extracted from

Chen, R.A.; Ryzhakov, G.; Cooray, S.; Randow, F.; Smith, G.L.
Inhibition of IkappaB kinase by vaccinia virus virulence factor B14 (2008), PLoS Pathog., 4, e22.

Search for more literature for 2.7.11.10

Protein Variants

Protein Variants	Comment	Organism
additional information	in cells infected with vaccina virus lacking gene B14R there is a higher level of phosphorylated IkappaBalpha but a similar level of IkappaBalpha compared to cells infected with control viruses expressing vaccina virus virulence factor B14, suggesting vaccina virus virulence factor B14 affects IKK activity	Homo sapiens
additional information	in cells infected with vaccinia virus lacking gene B14R there is a higher level of phosphorylated IkappaBalpha but a similar level of IkappaBalpha compared to cells infected with control viruses expressing vaccinia virus virulence factor B14, suggesting vaccinia virus virulence factor B14 affects IKK activity	Homo sapiens
S176E/S180E	vaccinia virus virulence factor B14 does inhibit a mutant containing a mutation in the activation loop. This suggests that phosphorylation of these serine residues in the activation loop of IKKbeta is targeted by B14	Homo sapiens
S177E/S181E	vaccina virus virulence factor B14 does not inhibit a mutant containing a mutation in the activation loop. This suggests that phosphorylation of these serine residues in the activation loop of IKKbeta is targeted by vaccina virus virulence factor B14	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
vaccina virus virulence factor B14	interacts and inhibits IKKcomplex. Interaction between vaccina virus virulence factor B14 and IKK complex requires IKKbeta but not IKKalpha	Homo sapiens
vaccinia virus virulence factor B14	interacts and inhibits IKKcomplex. Interaction between B14 and IKK complex requires IKKbeta but not IKKalpha	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293 cell	-	Homo sapiens	-
HeLa cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + KKKKERLLDDRHDSGLDSMKDEE	IKK substrate peptide derived from IkappaBalpha sequence	Homo sapiens	ADP + phosphorylated-KKKKERLLDDRHDSGLDSMKDEE	-	?

Synonyms

Synonyms	Comment	Organism
IkappaB kinase	-	Homo sapiens
IKKalpha	-	Homo sapiens
IKKbeta	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)