Application | Comment | Organism |
---|---|---|
molecular biology | IKKalpha and IKKbeta exert differential roles in ECM remodeling and endochondral ossification, which are events characteristic of hypertrophic chondrocytes and also complicating factors often found in osteoarthritic chondrocytes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of IKKalpha or IKKbeta is ablated in primary human chondrocytes by retrotransduction of specific short-hairpin RNAs. Micromass cultures designed to reproduce chondrogenesis with progression to the terminal hypertrophic stage are established, and anabolism and remodeling of the extracellular matrix (ECM) are investigated in the micromasses. Ablation of IKKalpha dramatically enhance type II collagen deposition independent of SOX9 protein levels but in association with suppressed levels of runt-related transcription factor 2. IKKalpha-deficient cells retain the phenotype of cells in a pre-hypertrophic-like state, as evidenced by the smaller size and faster proliferation of these cells prior to micromass seeding, along with the enhanced viability of their differentiated micromasses | Homo sapiens |
additional information | expression of IKKalpha or IKKbeta is ablated in primary human chondrocytes by retrotransduction of specific short-hairpin RNAs. Silencing of IKKbeta markedly enhances accumulation of glycosaminoglycan in conjunction with increased SOX9 expression | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14920 | - |
- |
Homo sapiens | O15111 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
chondrocyte | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
IKKalpha | - |
Homo sapiens |
IKKbeta | - |
Homo sapiens |