Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged wild-type and mutant enzymes in HEK-293 and HeLa cells, expression of the C-terminal fragment, comprising amino acids 541-716, of IKKepsilon with NF-kappaB2/p100/p52 in yeast two-hybrid cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K38A | inactive IKKepsilon mutant | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein p100 | Mus musculus | interaction with the NF-kappaB complex | ADP + phosphorylated protein p100 | - |
? | |
ATP + protein p165 | Mus musculus | p65 is part of the IKKepsilon complex with p25, interaction with the NF-kappaB complex | ADP + phosphorylated protein p165 | - |
? | |
additional information | Mus musculus | IKKepsilon is important in the regulation of the alternative NF-kappaB activation pathway involving p52 and p65, IKKepsilon interacts with p52 and promotes transactivation via p65 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein p100 | interaction with the NF-kappaB complex | Mus musculus | ADP + phosphorylated protein p100 | - |
? | |
ATP + protein p100 | required for the interaction with the NF-kappaB complex | Mus musculus | ADP + phosphorylated protein p100 | - |
? | |
ATP + protein p165 | p65 is part of the IKKepsilon complex with p25, interaction with the NF-kappaB complex | Mus musculus | ADP + phosphorylated protein p165 | - |
? | |
ATP + protein p165 | required for the interaction with the NF-kappaB complex | Mus musculus | ADP + phosphorylated protein p165 | - |
? | |
additional information | IKKepsilon is important in the regulation of the alternative NF-kappaB activation pathway involving p52 and p65, IKKepsilon interacts with p52 and promotes transactivation via p65 | Mus musculus | ? | - |
? | |
additional information | interaction analysis of IKKepsilon with NF-kappaB complex components/p25/p65 by immunoprecipitation and mass spectrometric analysis, overview | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IKKepsilon | - |
Mus musculus |
non-canonical IkappaBkinase epsilon | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |