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Literature summary for 2.7.11.10 extracted from

  • Gross, S.; Piwnica-Worms, D.
    Real-time imaging of ligand-induced IKK activation in intact cells and in living mice (2005), Nat. Methods, 2, 607-614.
    View publication on PubMed
Search for more literature for 2.7.11.10

Application

Application Comment Organism
analysis development of real-time imaging for continous enzyme detection and kinetics in intact cells and living mice utilizing a recombinant IkappaBalpha-firefly luciferase reporter construct, system can be used for determination of kinetics/pharmacodynamics of potential selective inhibitors, and for investigations of NF-kappaB signalling pathway activation Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [IkappaB protein] Mus musculus phosphorylation of IkappaB results in its proteolytic degradation ADP + [IkappaB phosphoprotein]
-
 ?
additional information Mus musculus IKK is required for activation of NF-kappaB and subsequent signalling pathways ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 development of real-time imaging for continous enzyme detection and kinetics in intact cells and living mice utilizing a recombinant IkappaBalpha-firefly luciferase reporter construct Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [IkappaB protein]
-
 Mus musculus ADP + [IkappaB phosphoprotein]
-
 ?
ATP + [IkappaB protein] phosphorylation of IkappaB results in its proteolytic degradation Mus musculus ADP + [IkappaB phosphoprotein]
-
 ?
additional information IKK is required for activation of NF-kappaB and subsequent signalling pathways Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
IKK
-
 Mus musculus