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Literature summary for 2.7.11.1 extracted from
- Functional characterization of receptor-interacting serine/threonine kinase 2 (RIP2) of the goldfish (Carassius auratus L.) (2015), Dev. Comp. Immunol., 48, 76-85 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RIPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative expression analysis, cloning and recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 | Carassius auratus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibitin by gefitinib and erlotinib. DMSO at up to 0.1% has no effect on enzyme activity | Carassius auratus | |
| SB203580 | the p38 MAPK pathway inhibitor inhibits enzyme activity of RIP2 | Carassius auratus |  |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | at up to 0.1% has no effect on enzyme activity | Carassius auratus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carassius auratus | A0A0F6PAW6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Carassius auratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | higher expression level | Carassius auratus | - |
| gill | - |
Carassius auratus | - |
| heart | low enzyme level | Carassius auratus | - |
| intestine | - |
Carassius auratus | - |
| kidney | - |
Carassius auratus | - |
| liver | very low enzyme level | Carassius auratus | - |
| macrophage | - |
Carassius auratus | - |
| monocyte | - |
Carassius auratus | - |
| additional information | tissue-specific quantitative expression analysis indicates the highest mRNA levels of RIP2 in the spleen, followed by brain, intestine, gill, and kidney | Carassius auratus | - |
| muscle | low enzyme level | Carassius auratus | - |
| neutrophil | - |
Carassius auratus | - |
| spleen | very high expression level | Carassius auratus | - |
| splenocyte | - |
Carassius auratus | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65700, about, sequence calculation | Carassius auratus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| receptor-interacting serine/threonine kinase 2 | - |
Carassius auratus |
| RIP2 | - |
Carassius auratus |
| RIPK2 | - |
Carassius auratus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Carassius auratus | sequence calculation | - |
6.05 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Carassius auratus | RIP2 is upregulated by lipopolysaccharide, peptidoglycan, and muramyl dipeptide treatment after 6 h, and by all Nod1/Nod2 ligands after 12 h. The enzyme expression is also upregulated by heat-killed Aeromonas salmonicida, and heat-killed or live Mycobacterium marinum | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | RIP2 is a critical protein involved in the modulation of macrophage pro-inflammatory responses in teleosts. RIP2 overexpression causes the activation of the NF-?B signal pathway | Carassius auratus |
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Release 2023.1 (January 2023)
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