Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + SREBP-1c | Rattus norvegicus | sterol response element binding protein-1c, phosphorylation of Ser73 | ADP + phospho-SREBP-1c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P18266 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatocyte | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + SREBP-1c | sterol response element binding protein-1c, phosphorylation of Ser73 | Rattus norvegicus | ADP + phospho-SREBP-1c | - |
? | |
ATP + SREBP-1c | sterol response element binding protein-1c phosphorylation of Ser73 | Rattus norvegicus | ADP + phospho-SREBP-1c | - |
? | |
additional information | recombinant expression of His6- or HA-tagged full-length substrate SREBP-1c and its loss-of-function mutant S73A and gain-of-function mutant S73D in McA-RH7777 cells, overview. The sequence surrounding serine 73 of rat SREBP-1c resembles a canonical cdc4 phosphodegron (CPD) that is a recognized substrate of the SCFFbw7 ubiquitin ligase | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycogen synthase kinase-3 | - |
Rattus norvegicus |
GSK-3 | - |
Rattus norvegicus |
GSK-3beta | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition or knockdown of GSK-3 differentially regulates the turnover of wild-type and S73A SREBP-1c proteins | Rattus norvegicus |
physiological function | glycogen synthase kinase-3-mediated phosphorylation of serine 73 targets sterol response element binding protein-1c (SREBP-1c) for ubiquitination and proteasomal degradation. Also phospho-mimetic mutation of serine 73 targets SREBP-1c for proteasomal degradation. Constitutive phosphorylation at serine 73 (as is the case for the S73D mutant of SREBP-1c) results in reduced affinity of the pSREBPSCAP complex in the endoplasmic reticulum. The S73D pSREBP-1c mutant becomes free and is targeted for rapid proteasomal degradation | Rattus norvegicus |