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Literature summary for 2.7.11.1 extracted from
- Structure-function study of deinococcal serine/threonine protein kinase implicates its kinase activity and DNA repair protein phosphorylation roles in radioresistance of Deinococcus radiodurans (2013), Int. J. Biochem. Cell Biol., 45, 2541-2552.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) pLysS cells | Deinococcus radiodurans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K42A | inactive | Deinococcus radiodurans |
| S171A | the mutant shows about 10% of wild type activity | Deinococcus radiodurans |
| T169A | the mutant shows about 5% of wild type activity | Deinococcus radiodurans |
| T169A/S171A | the mutant shows less than 1% of wild type activity | Deinococcus radiodurans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + PprA protein | Deinococcus radiodurans | phosphorylation at threonine 72, serine 112, and threonine 144 | ADP + PprA phosphoprotein | - |
? |  |
| ATP + PprA protein | Deinococcus radiodurans ATCC 13939 | phosphorylation at threonine 72, serine 112, and threonine 144 | ADP + PprA phosphoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | Q9RRH3 | - |
- |
| Deinococcus radiodurans ATCC 13939 | Q9RRH3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + PprA protein | phosphorylation at threonine 72, serine 112, and threonine 144 | Deinococcus radiodurans | ADP + PprA phosphoprotein | - |
? | |
| ATP + PprA protein | phosphorylation at threonine 72, serine 112, and threonine 144 | Deinococcus radiodurans ATCC 13939 | ADP + PprA phosphoprotein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DR2518 | locus name | Deinococcus radiodurans |
| RqkA | - |
Deinococcus radiodurans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | serine/threonine phosphorylation of PprA protein by the enzyme supports its role in radioresistance of Deinococcus radiodurans | Deinococcus radiodurans |
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