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Literature summary for 2.7.11.1 extracted from

  • Lebska, M.; Ciesielski, A.; Szymona, L.; Godecka, L.; Lewandowska-Gnatowska, E.; Szczegielniak, J.; Muszynska, G.
    Phosphorylation of maize eukaryotic translation initiation factor 5A (eIF5A) by casein kinase 2: Identification of phosphorylated residue and influence on intracellular localization of eIF5A (2010), J. Biol. Chem., 285, 6217-6226.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
cloning of catalytic subunit alpha of CK2, i.e. ZmCK2alpha-1, and expression as GST-tagged enzyme in Escherichia coli strain BL21(DE3) Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Zea mays 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + maize eukaryotic translation initiation factor 5A Zea mays
-
 ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?
ATP + maize eukaryotic translation initiation factor 5A Zea mays Mona
-
 ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-
Zea mays Mona
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native CK2 from seedlings by ammonium sulfate fractionation, cation exchange chromatography, ultrafiltration, dialysis, anion exchange chromatography, again ultrafiltration, and gel filtration Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Zea mays
-
seedling
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + maize eukaryotic translation initiation factor 5A
-
 Zea mays ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?
ATP + maize eukaryotic translation initiation factor 5A phosphorylation at Ser2 by CK2. Zea mays, Arabidopsis thaliana, and Saccharomyces cerevisiae eIF5A wild-type proteins are efficient substrates, while their S2A mutants are not phosphorylated, the S4A mutant is phosphorylated Zea mays ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?
ATP + maize eukaryotic translation initiation factor 5A
-
 Zea mays Mona ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?
ATP + maize eukaryotic translation initiation factor 5A phosphorylation at Ser2 by CK2. Zea mays, Arabidopsis thaliana, and Saccharomyces cerevisiae eIF5A wild-type proteins are efficient substrates, while their S2A mutants are not phosphorylated, the S4A mutant is phosphorylated Zea mays Mona ADP + phosphorylated maize eukaryotic translation initiation factor 5A
-
 ?

Synonyms

Synonyms Comment Organism
casein kinase 2
-
 Zea mays
CK2
-
 Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Zea mays

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Zea mays

General Information

General Information Comment Organism
physiological function phosphorylation of Ser2 plays a role in regulation of nucleocytoplasmic shuttling of eukaryotic translation initiation factor 5A in plant cells Zea mays