Literature summary for 2.7.11.1 extracted from

Ragan, T.J.; Ross, D.B.; Keshwani, M.M.; Harris, T.K.
Expression, purification, and characterization of a structurally disordered and functional C-terminal autoinhibitory domain (AID) of the 70 kDa 40S ribosomal protein S6 kinase-1 (S6K1) (2008), Protein Expr. Purif., 57, 271-279.

Protein Variants

Protein Variants	Comment	Organism
DELTA1-398	the autoinhibitory domain is separately expressed and purified. Trans-addition of purified AID effectively inhibits PDK1-catalyzed T-loop phosphorylation of a catalytic kinase domain construct of S6K1	Homo sapiens
DELTA1-398/S411D/S418D/T421E/S424D	expression of the autoinhibitory domain with several mutations shows that the tetraphospho-mimicking mutations in the autoinhibitory domain construct significantly decreased AID-mediated inhibition of PDK1-catalyzed T229 phosphorylation of DELTA399-502/T389E (His6-S6K1(DELTA AID)-T389E)	Homo sapiens
DELTA399-502/T389E	by using a mutant protein wich lacks the autoinhibitory domain (AID) and which contains a T389E mutation it is shown that the autoinhibiotry domain (AID) of S6K1 expressed separately most significantly inhibits PDK1-catalyzed T229 phosphorylation in the T-loop of the S6K1 catalytic kinase domain construct, His6-S6K1(DELTA AID)-T389E	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
auoinhibitory domain of S6K1	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P23443	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	catalytic activation requires dual phosphorylation of critical residues in the conserved T-loop (T229) and hydrophobic motif (HM, T389) peptide regions of its catalytic kinase domain (residues 1-398)	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
optimal bacterial expression and affinity purification of the full autoinhibitory domain (AID) of S6K1 is achieved by fusing GST to the N-terminus, growing the BL21 (DE3) strain of Escherichia coli in PG minimal medium, adding the irreversible protease inhibitors TPCK and TLCK to the lysis buffer, using the highly selective PreScission Protease to remove the GST affinity tag, and using the Superdex 75 size-exclusion column to resolve cleaved full length AID from smaller proteolyzed fragments	Homo sapiens

Purification (Comment)

Organism

optimal bacterial expression and affinity purification of the full autoinhibitory domain (AID) of S6K1 is achieved by fusing GST to the N-terminus, growing the BL21 (DE3) strain of Escherichia coli in PG minimal medium, adding the irreversible protease inhibitors TPCK and TLCK to the lysis buffer, using the highly selective PreScission Protease to remove the GST affinity tag, and using the Superdex 75 size-exclusion column to resolve cleaved full length AID from smaller proteolyzed fragments

Homo sapiens

Synonyms

Synonyms	Comment	Organism
S6K1	-	Homo sapiens
serine-threonine protein kinase	-	Homo sapiens