Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
GTP | autophosphorylation | Aquifex aeolicus | |
0.64 | - |
ATP | autophosphorylation | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
SDS-PAGE | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66844 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | enzyme is autophosphorylated on a threonine residue | Aquifex aeolicus |
Purification (Comment) | Organism |
---|---|
using Ni2+-affinity chromatography | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AaSTPK | enzyme is autophosphorylated on a threonine residue | Aquifex aeolicus | ADP + phosphorylated-AaSTPK | - |
? | |
ATP + CPS.A subunit | AaSTPK also phosphorylates one threonine residue on the carbamoyl phosphate synthetase, CPS.A subunit | Aquifex aeolicus | ADP + phosphorylated-CPS.A subunit | - |
? | |
GTP + AaSTPK | enzyme is autophosphorylated on a threonine residue | Aquifex aeolicus | GDP + phosphorylated-AaSTPK | - |
? | |
GTP + CPS.A subunit | - |
Aquifex aeolicus | GDP + phosphorylated-CPS.A subunit | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
AaSTPK | - |
Aquifex aeolicus |
serine-threonine kinase | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
GTP | autophosphorylation | Aquifex aeolicus | |
0.00016 | - |
ATP | autophosphorylation | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
MgATP2- | preferred substrate | Aquifex aeolicus | |
MnATP2- | - |
Aquifex aeolicus | |
MnGTP2- | - |
Aquifex aeolicus |