Literature summary for 2.7.11.1 extracted from

Yan, Y.; Nguyen, H.; Dalmasso, G.; Sitaraman, S.V.; Merlin, D.
Cloning and characterization of a new intestinal inflammation-associated colonic epithelial Ste20-related protein kinase isoform (2007), Biochim. Biophys. Acta, 1769, 106-116.

Search for more literature for 2.7.11.1

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	expression of the intestinal inflammation-associated colonic epithelial SPAK isozyme is upregulated by the pro-inflammatory cytokine IFN-gamma, but not by TGF-beta and TNF-alpha, overview	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, in vitro transcription and translation, overexpression of the SPAK isozyme in Caco-2-BBE cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
I34L	site-directed mutagenesis, inactive mutant of the intestinal inflammation-associated colonic epithelial isozyme	Homo sapiens
additional information	overexpression of the SPAK isozyme leads to leading to nuclear translocation of an N-terminal fragment of the SPAK isozyme, as well as activation of p38 MAP kinase signaling cascades and increased intestinal barrier permeability	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52600	-	x * 52600, about, sequence calculation, x * 53000, in vitro expressed enzyme, SDS-PAGE, x * 60000, about, enzyme expressed in Caco-2-BBE cells, SDS-PAGE	Homo sapiens
53000	-	x * 52600, about, sequence calculation, x * 53000, in vitro expressed enzyme, SDS-PAGE, x * 60000, about, enzyme expressed in Caco-2-BBE cells, SDS-PAGE	Homo sapiens
60000	-	x * 52600, about, sequence calculation, x * 53000, in vitro expressed enzyme, SDS-PAGE, x * 60000, about, enzyme expressed in Caco-2-BBE cells, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the enzyme is involved in the inflammation cascade in intestine and colon epithelium	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q6E0B2	small intestine SPAK-like kinase; intestinal inflammation-associated colonic epithelial isozyme	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
colon	epithelium, inflamed and non-inflamed tissue	Homo sapiens	-
epithelium	-	Homo sapiens	-
intestine	-	Homo sapiens	-
additional information	no activity of the intestinal inflammation-associated colonic epithelial isozyme in liver, spleen, brain, prostate, and kidney	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + myelin basic protein	-	Homo sapiens	ADP + phosphorylated myelin basic protein	-	?
additional information	the enzyme is involved in the inflammation cascade in intestine and colon epithelium	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 52600, about, sequence calculation, x * 53000, in vitro expressed enzyme, SDS-PAGE, x * 60000, about, enzyme expressed in Caco-2-BBE cells, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
serine/threonine kinase	-	Homo sapiens
SPAK/STE20	-	Homo sapiens
SPS1-related proline alanine-rich kinase	-	Homo sapiens
Ste20-related protein kinase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	sequence calculation	-	6.51

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Release 2023.1 (January 2023)