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Literature summary for 2.7.11.1 extracted from

  • Cavet, M.E.; Lehoux, S.; Berk, B.C.
    14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity (2003), J. Biol. Chem., 278, 18376-18383.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information RSK1 stimulation by EGF via CREB, a cAMP-response element-binding protein, autophosphorylation at Ser363 and Ser380 of RSK1 and membrane translocation are required for full RSK1 activation Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
in vitro transcription and translation of RSK1, expression of RSK1 in PS127A cells or in HEK-293 cells, coexpression and colocalization in cytoplasm of FLAG-tagged RSK1 and 14-3-3beta in COS-7 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
S154A site-directed mutagenesis, mutation modulates the enzyme activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
14-3-3beta a p90 ribosomal S6 kinase isoform 1-binding protein that negatively regulates RSK1 kinase activity, interaction via Lys49 of 14-3-3beta and S154 of phosphorylated RSK1, phosphorylation of RSK1 is required for interaction, no binding of mutant 14-3-3beta K49Q or K49A Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Rattus norvegicus 5737
-
plasma membrane membrane translocation is required for full RSK1 activation Rattus norvegicus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Rattus norvegicus
Mn2+
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus isozyme RSK1, i.e. 14-3-3, is regulated by inhibitory binding of 14-3-3beta, a RSK1-binding protein, to Lys49 of the enzyme, Lys49 is also responsible for interaction of RSK1 with Bcr, Raf, and Cbl ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
isozyme RSK1, i.e. p90 ribosomal S6 kinase or 14-3-3
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein RSK1 is autophosphorylated at Ser363 and Ser380 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isozyme RSK1, i.e. 14-3-3, is regulated by inhibitory binding of 14-3-3beta, a RSK1-binding protein, to Lys49 of the enzyme, Lys49 is also responsible for interaction of RSK1 with Bcr, Raf, and Cbl Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
14-3-3
-
Rattus norvegicus
p90 ribosomal S6 kinase
-
Rattus norvegicus
RSK
-
Rattus norvegicus
RSK1
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Rattus norvegicus