Literature summary for 2.7.11.1 extracted from

Chen, X.; Lin, G.; Wei, Y.; Hexige, S.; Niu, Y.; Liu, L.; Yang, C.; Yu, L.
TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway (2005), Biochem. Biophys. Res. Commun., 333, 742-749.

Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis of TSSK5, expression of His-tagged CREB in Escherichia coli, expression of GST-fusion TSSK5 in HEK-293 cells, co-expression of TSSK5 and CREB in Saccharomyces cerevisiae using the two-hydrid system	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + CREB	Homo sapiens	phosphorylation by TSSK5 at Ser133 stimulates the cAMP responsive element/cAMP responsive element binding protein CRE/CREB responsive pathway in recombinant HEK293 cells	ADP + phosphorylated CREB	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q6SA08	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-fusion TSSK5 from HEK-293 cells by glutathione affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
testis	TSSK5, absolutely testis-specific	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + CREB	phosphorylation by TSSK5 at Ser133 stimulates the cAMP responsive element/cAMP responsive element binding protein CRE/CREB responsive pathway in recombinant HEK293 cells	Homo sapiens	ADP + phosphorylated CREB	-	?
ATP + CREB	i.e. cAMP responsive element binding protein, phosphorylation by TSSK5 at Ser133, no activity with CREB S133A mutant	Homo sapiens	ADP + phosphorylated CREB	-	?

Synonyms

Synonyms	Comment	Organism
More	TSSK5 is a member of the testis-specific serine/threonine kinase family	Homo sapiens
testis-specific serine/threonine kinase	-	Homo sapiens
TSSK5	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)