Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation, can replace DTT | Oncorhynchus mykiss | |
3',5'-AMP | - |
Oncorhynchus mykiss |
General Stability | Organism |
---|---|
freeze-thawing inactivates | Oncorhynchus mykiss |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | not | Oncorhynchus mykiss | |
high ionic strength | activation, e.g. NaCl, NH4Cl, KCl, sodium acetate, essential for maximal phosphate incorporation with protamine as substrate, histone phosphorylation is suppressed | Oncorhynchus mykiss | |
Mg2+ | requirement | Oncorhynchus mykiss | |
Mn2+ | can replace Mg2+ with 8% efficiency | Oncorhynchus mykiss | |
additional information | no activation by Ca2+ or Zn2+ | Oncorhynchus mykiss |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oncorhynchus mykiss | - |
rainbow trout | - |
Purification (Comment) | Organism |
---|---|
partial | Oncorhynchus mykiss |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Oncorhynchus mykiss | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.07 | - |
- |
Oncorhynchus mykiss |
Storage Stability | Organism |
---|---|
0-4°C, partially purified preparation, several weeks | Oncorhynchus mykiss |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | poor substrate | Oncorhynchus mykiss | ? | - |
? | |
ATP + histone fraction II | slightly lysine-rich, from trout testes | Oncorhynchus mykiss | ? | - |
? | |
ATP + protamine | preferred substrate | Oncorhynchus mykiss | ADP + O-phosphoprotamine | - |
? | |
ATP + protamine | transfers terminal phosphoryl group from ATP into O-phosphoseryl linkages in the acceptor molecule | Oncorhynchus mykiss | ADP + O-phosphoprotamine | - |
? | |
ATP + protamine | no substrates are GTP | Oncorhynchus mykiss | ADP + O-phosphoprotamine | - |
? | |
ATP + protamine | no substrates are GTP or UTP | Oncorhynchus mykiss | ADP + O-phosphoprotamine | - |
? | |
ATP + protamine | phosphorylates histone fraction I (lysine-rich) and histone fractions III and IV (arginine-rich), from trout testes | Oncorhynchus mykiss | ADP + O-phosphoprotamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Oncorhynchus mykiss |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | at high salt concentration | Oncorhynchus mykiss |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 8.6 | about half-maximal activity at pH 6.4 and about 65% of maximal activity at pH 8.6, at high salt concentration | Oncorhynchus mykiss |