Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.10.2 extracted from

  • Xiong, X.; Cui, P.; Hossain, S.; Xu, R.; Warner, B.; Guo, X.; An, X.; Debnath, A.K.; Cowburn, D.; Kotula, L.
    Allosteric inhibition of the nonMyristoylated c-Abl tyrosine kinase by phosphopeptides derived from Abi1/Hssh3bp1 (2008), Biochim. Biophys. Acta, 1783, 737-747.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged, partially capped, active, nonmyristoylated c-Abl, E46 through C-terminus, isoform 1b numbering, is produced, GST fusions of c-Abl SH3 and SH2 domains are used, the dual domain SH3-SH2 is expressed from plasmid pTXB1 Homo sapiens

Protein Variants

Protein Variants Comment Organism
R171K SH2 domain variant containing a mutation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information pY213 phosphopeptide of Abi1 inhibits c-Abl kinase activity by a noncompetitive mechanism Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [protein]-L-tyrosine Homo sapiens
-
ADP + [protein]-L-tyrosine phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity purified on nickel-nitriloacetic acid agarose and by ion-exchange chromatography using a Mono S column, through chitin affinitive binding and by SP Sepharose cation exchange Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GGEAIYAAPFKK kinase assay using biotinylated model substrate peptide Homo sapiens ADP + GGEAIYAAPFKK phosphate
-
?
ATP + [Abi1]-L-tyrosine c-Abl phosphorylates Y213 of Abi1 Homo sapiens ADP + [Abi1]-L-tyrosine phosphate
-
?
ATP + [protein]-L-tyrosine
-
Homo sapiens ADP + [protein]-L-tyrosine phosphate
-
?

Synonyms

Synonyms Comment Organism
c-Abl tyrosine kinase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
kinase assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
kinase assay Homo sapiens