Cloned (Comment) | Organism |
---|---|
His-tagged, partially capped, active, nonmyristoylated c-Abl, E46 through C-terminus, isoform 1b numbering, is produced, GST fusions of c-Abl SH3 and SH2 domains are used, the dual domain SH3-SH2 is expressed from plasmid pTXB1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R171K | SH2 domain variant containing a mutation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | pY213 phosphopeptide of Abi1 inhibits c-Abl kinase activity by a noncompetitive mechanism | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-L-tyrosine | Homo sapiens | - |
ADP + [protein]-L-tyrosine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity purified on nickel-nitriloacetic acid agarose and by ion-exchange chromatography using a Mono S column, through chitin affinitive binding and by SP Sepharose cation exchange | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GGEAIYAAPFKK | kinase assay using biotinylated model substrate peptide | Homo sapiens | ADP + GGEAIYAAPFKK phosphate | - |
? | |
ATP + [Abi1]-L-tyrosine | c-Abl phosphorylates Y213 of Abi1 | Homo sapiens | ADP + [Abi1]-L-tyrosine phosphate | - |
? | |
ATP + [protein]-L-tyrosine | - |
Homo sapiens | ADP + [protein]-L-tyrosine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
c-Abl tyrosine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
kinase assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
kinase assay | Homo sapiens |