Cloned (Comment) | Organism |
---|---|
overexpression of Myc-tagged wild-type non-receptor tyrosine kinase Pyk2 and mutant enzyme forms in 293T cells, wild-type enzyme expression leads to increased activation of Stat5B, cotransfection with wild-type FGFR1-4, FGFR3 mutant K650E, a myristoylated FGFR, and truncated FGFR3 in 293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K457A | site-directed mutagenesis, mutation leads to increase in phosphorylation sites on the substrate FGFR3 | Homo sapiens |
additional information | construction of a Pyk2 mutant Pyk2-DELTAKIN lacking the kinase domain resulting in loss of interaction and phosphorylation activity with FGFR3 | Homo sapiens |
Y402F | site-directed mutagenesis, mutation leads to increase in phosphorylation sites on the substrate FGFR3 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [fibroblast growth factor receptor FGFR2]-L-tyrosine | Homo sapiens | activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain, activation is antagonized by tyrosine phosphatase Shp2 | ADP + [fibroblast growth factor receptor FGFR2]-L-tyrosine phosphate | - |
? | |
ATP + [fibroblast growth factor receptor FGFR3]-L-tyrosine | Homo sapiens | activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain, activation is antagonized by tyrosine phosphatase Shp2 | ADP + [fibroblast growth factor receptor FGFR3]-L-tyrosine phosphate | - |
? | |
ATP + [Stat5B]-L-tyrosine | Homo sapiens | activation by non-receptor tyrosine kinase Pyk2 | ADP + [Stat5B]-L-tyrosine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Pyk2 performs essential autophosphorylation at Tyr402, this phosphorylation step is not essential in case of FRGFR3 activation by phosphorylation through Pyk2, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [fibroblast growth factor receptor FGFR2]-L-tyrosine | activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain, activation is antagonized by tyrosine phosphatase Shp2 | Homo sapiens | ADP + [fibroblast growth factor receptor FGFR2]-L-tyrosine phosphate | - |
? | |
ATP + [fibroblast growth factor receptor FGFR2]-L-tyrosine | activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain | Homo sapiens | ADP + [fibroblast growth factor receptor FGFR2]-L-tyrosine phosphate | - |
? | |
ATP + [fibroblast growth factor receptor FGFR3]-L-tyrosine | activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain, activation is antagonized by tyrosine phosphatase Shp2 | Homo sapiens | ADP + [fibroblast growth factor receptor FGFR3]-L-tyrosine phosphate | - |
? | |
ATP + [fibroblast growth factor receptor FGFR3]-L-tyrosine | activation by non-receptor tyrosine kinase Pyk2, interaction via the FGFR juxtamembrane and the Pyk2 kinase domain | Homo sapiens | ADP + [fibroblast growth factor receptor FGFR3]-L-tyrosine phosphate | - |
? | |
ATP + [Stat5B]-L-tyrosine | activation by non-receptor tyrosine kinase Pyk2 | Homo sapiens | ADP + [Stat5B]-L-tyrosine phosphate | - |
? | |
additional information | Pyk2 performs autophosphorylation at Tyr402, fibroblast growth factor receptors FGFR1 and FGFR4 are no substrates of Pyk2 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
non-receptor protein-tyrosine kinase | - |
Homo sapiens |
proline-rich tyrosin ekinase 2 | - |
Homo sapiens |
Pyk2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |