Literature summary for 2.7.10.2 extracted from

Mahabeleshwar, G.H.; Kundu, G.C.
Tyrosine kinase p56lck regulates cell motility and nuclear factor kappaB-mediated secretion of urokinase type plasminogen activator through tyrosine phosphorylation of IkappaBalpha following hypoxia/reoxygenation (2003), J. Biol. Chem., 278, 52598-52612.

Search for more literature for 2.7.10.2

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	hypoxia/reoxygenation induces tyrosine phosphorylation of Lck and Lck activity in breast cancer cells and of IkappaBalpha, but not of IkappaBalpha serine phosphorylation, activation is inhibited by emodin and pp2	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type Lck in Y394F Lck mutant cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
Y394F	Lck F394 mutant cells show reduced tyrosine phosphorylation of the enzyme, expression of recombinant wild-type Lck in the mutant cells increases the autophosphorylation activity 3fold	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
4-amino-5-(4-chlorophenyl)-7-(tert-butyl)pyrazolo[3,4-d]pyrimidine	i.e. pp2, Lck inhibitor	Homo sapiens
emodin	i.e. 6-methyl-1,3,8-trihydroxyanthraquinone, Lck inhibitor	Homo sapiens
additional information	regulation of Lck by phosphorylation at Tyr505 by C-terminal Scr protein-tyrosine kinase which inhibits the enzyme	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens
Mn2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + IkappaBalpha-L-tyrosine	Homo sapiens	p56lck regulates cell motility and nuclear factor kappaB-mediated secretion of urokinase type plasminogen activator through tyrosine phosphorylation of IkappaBalpha following hypoxia/reoxygenation, molecular mechanism, physiological role	ADP + IkappaBalpha-L-tyrosine phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	regulation of Lck by phosphorylation at Tyr505 by C-terminal Scr protein-tyrosine kinase which inhibits the enzyme	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
breast cancer cell	-	Homo sapiens	-
MCF-7 cell	low invasive breast cancer cell	Homo sapiens	-
MDA-MB-231 cell	high invasive breast cancer cell	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + IkappaBalpha-L-tyrosine	p56lck regulates cell motility and nuclear factor kappaB-mediated secretion of urokinase type plasminogen activator through tyrosine phosphorylation of IkappaBalpha following hypoxia/reoxygenation, molecular mechanism, physiological role	Homo sapiens	ADP + IkappaBalpha-L-tyrosine phosphate	-	?
ATP + IkappaBalpha-L-tyrosine	Lck interacts via its SH2 domain with tyrosine-phosphorylated IkappaBalpha	Homo sapiens	ADP + IkappaBalpha-L-tyrosine phosphate	-	?

Synonyms

Synonyms	Comment	Organism
Lck	-	Homo sapiens
More	Lck is a member of the Src non-receptor tyrosine kinase family	Homo sapiens
p56lck	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.7	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)