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Literature summary for 2.7.10.2 extracted from

  • Nore, B.F.; Mattsson, P.T.; Antonsson, P.; Backesjo, C.M.; Westlund, A.; Lennartsson, J.; Hansson, H.; Low, P.; Ronnstrand, L.; Smith, C.I.
    Identification of phosphorylation sites within the SH3 domains of Tec family tyrosine kinases (2003), Biochim. Biophys. Acta, 1645, 123-132.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information Tec family tyrosine kinase is activated by tyrosine phosphorylation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of SH3 domains of Tec tyrosine kinase family members in Escherichia coli strain XL-1 Blue as GST-fusion proteins, expression of His-tagged SH3 domain from Btk in Spodoptera frugiperda Sf9 cells using a baculovirus expression system, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y223A site-directed mutagenesis of the SH3 domain tyrosine of Btk, the mutant is not phosphorylated Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Mn2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a [protein]-L-tyrosine Homo sapiens
-
ADP + a [protein]-L-tyrosine phosphate
-
?
additional information Homo sapiens Tec family tyrosine kinases play a central role in hematopoietic cellular signaling ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein Tec family tyrosine kinase is activated by tyrosine phosphorylation in the activation loop, a transphosphorylation mechanism is used for further acivation in the SH3 domain, specific phosphorylation at conserved residues, i.e. Tyr223 of Btk, Tyr180 of Itk, Tyr215 of Bmx, and Tyr206 of Tec, autophosphorylation or phosphorylation by family members, but not of other non-family SH3-domains, proline-rich consensus sequences, overview Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion-SH3 domains of Tec tyrosine kinase family members from Escherichia coli strain XL-1 Blue, recombinant His-tagged Btk SH3 domain from Sf9 cells, affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a [protein]-L-tyrosine
-
Homo sapiens ADP + a [protein]-L-tyrosine phosphate
-
?
ATP + a [protein]-L-tyrosine enzymes of the Tec tyrosine kinase family phosphorylate themselves or other family members Homo sapiens ADP + a [protein]-L-tyrosine phosphate
-
?
additional information Tec family tyrosine kinases play a central role in hematopoietic cellular signaling Homo sapiens ?
-
?
additional information site specificity of tyrosine phosphorylation by Tec family members, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More SH3 domain structures, ligand binding structures Homo sapiens

Synonyms

Synonyms Comment Organism
Bmx
-
Homo sapiens
Bruton's tyrosine kinase
-
Homo sapiens
Btk
-
Homo sapiens
Itk
-
Homo sapiens
More Tec, Btk, Itk, and Bmx are members of the Tec tyrosine kinase family Homo sapiens
Tec
-
Homo sapiens
Tec family tyrosine kinase
-
Homo sapiens
TFK
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens