Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged platelet-derived growth factor receptor-beta wild-type and mutants in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y740F/Y751F | site-directed mutagenesis, reduced activity with GRK2 compared to the wild-type enzyme, the mutant cannot recruit phosphoinositide 3-kinase | Homo sapiens |
Y857F | site-directed mutagenesis, mutant shows reduced sensitivity to GRK2 inhibitory phosphorylation compared to the wild-type PDGFRbeta | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
G protein-coupled receptor kinase-2 | platelet-derived growth factor-dependent GRK2 phosphorylation desensitizes the PDGF receptor-beta | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + G protein-coupled receptor kinase-2 | Homo sapiens | GRK2 activation also increases GRK2 degradation and downregulation, independent of Gbetagamma subunits and phosphoinositide 3-kinase | ADP + phosphorylated G protein-coupled receptor kinase-2 | - |
? | |
additional information | Homo sapiens | PDGFRbeta wild-type and mutant Y857F activate c-Src kinase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | PDGFRbeta is phosphorylated by GRK2, and autophosphorylated | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + G protein-coupled receptor kinase-2 | GRK2 activation also increases GRK2 degradation and downregulation, independent of Gbetagamma subunits and phosphoinositide 3-kinase | Homo sapiens | ADP + phosphorylated G protein-coupled receptor kinase-2 | - |
? | |
ATP + G protein-coupled receptor kinase-2 | activates GRK2 by tyrosine phosphorylation at Y13, Y86, and Y92, the wild-type PDGFRbeta is 60fold more active with GRK2 than PDGFRbeta mutant Y857F, independent of Gbetagamma subunits and phosphoinositide 3-kinase | Homo sapiens | ADP + phosphorylated G protein-coupled receptor kinase-2 | - |
? | |
additional information | PDGFRbeta wild-type and mutant Y857F activate c-Src kinase | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDGFRbeta | - |
Homo sapiens |
platelet-derived growth factor receptor-beta | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |