Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
cytidine | pH 7.0, 50°C, cosubstrate: ATP | Methanocaldococcus jannaschii | |
0.021 | - |
Inosine | pH 7.0, 50°C, cosubstrate: ATP | Methanocaldococcus jannaschii | |
0.062 | - |
guanosine | pH 7.0, 70°C, cosubstrate: ATP | Methanocaldococcus jannaschii | |
0.078 | - |
guanosine | pH 7.0, 50°C, cosubstrate: ATP | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%) | Methanocaldococcus jannaschii | |
Mg2+ | enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%) | Methanocaldococcus jannaschii | |
Mn2+ | enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%) | Methanocaldococcus jannaschii | |
Ni2+ | enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%) | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Methanocaldococcus jannaschii |
68000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanocaldococcus jannaschii | the enzyme plays a role in nucleotide metabolism | ? | - |
? | |
additional information | Methanocaldococcus jannaschii DSM 2661 | the enzyme plays a role in nucleotide metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57849 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q57849 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cytidine | at 70°C the Vmax/Km-value for cytidine is 93% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for cytidine is 59% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP | Methanocaldococcus jannaschii | ADP + CMP | - |
? | |
ATP + cytidine | at 70°C the Vmax/Km-value for cytidine is 93% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for cytidine is 59% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP | Methanocaldococcus jannaschii DSM 2661 | ADP + CMP | - |
? | |
ATP + guanosine | at 70°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP | Methanocaldococcus jannaschii | ADP + GMP | - |
? | |
ATP + guanosine | at 70°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP | Methanocaldococcus jannaschii DSM 2661 | ADP + GMP | - |
? | |
ATP + inosine | relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) | Methanocaldococcus jannaschii | ADP + IMP | - |
? | |
ATP + inosine | relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) | Methanocaldococcus jannaschii DSM 2661 | ADP + IMP | - |
? | |
GTP + cytidine | relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) | Methanocaldococcus jannaschii | GDP + CMP | - |
? | |
GTP + guanosine | relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) | Methanocaldococcus jannaschii | GDP + GMP | - |
? | |
GTP + inosine | relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) | Methanocaldococcus jannaschii | GDP + IMP | - |
? | |
ITP + cytidine | relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) | Methanocaldococcus jannaschii | IDP + CMP | - |
? | |
ITP + guanosine | relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) | Methanocaldococcus jannaschii | IDP + GMP | - |
? | |
ITP + inosine | relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%) | Methanocaldococcus jannaschii | IDP + IMP | - |
? | |
additional information | the enzyme plays a role in nucleotide metabolism | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | adenosine, xanthosine, 2'-deoxyadenosine, uridine, and thymidine are used at low rates, the enzyme catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate at an extremely low rate | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | the enzyme plays a role in nucleotide metabolism | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? | |
additional information | adenosine, xanthosine, 2'-deoxyadenosine, uridine, and thymidine are used at low rates, the enzyme catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate at an extremely low rate | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
MJ0406 | locus name | Methanocaldococcus jannaschii |
NTP:nucleoside kinase | - |
Methanocaldococcus jannaschii |
PFK-B sugar kinase | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
melting temperature | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.1 | 8.2 | 50% of maximal activity at pH 6.1 and at pH 8.2 | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays a role in nucleotide metabolism | Methanocaldococcus jannaschii |