Crystallization (Comment) | Organism |
---|---|
sitting- and hanging-drop vapour diffusion methods, three-dimensional structures of the unliganded enzyme and a complex of the enzyme, an ATP analogue and adenosine are determined to 1.7 and 1.9 A resolution, respectively. In the crystal structure of the MjNK complex, subunit A adopts a closed conformation and subunit B an open conformation. In subunit A all substrates and Mg2+ are observed, whereas in subunit B only the ATP analogue can be clearly identified in the electron density | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | there are two magnesium-binding sites per subunit: one Mg2+ (MO6) is coordinated octahedrally by six water molecules and is located between adenosine and AMPPNP in the active site and one Mg2+ (MO5) is coordinated by five water molecules and is positioned in the interface between the dimers in the crystal | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57849 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q57849 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
MJ0406 | locus name | Methanocaldococcus jannaschii |
MjNK | - |
Methanocaldococcus jannaschii |
nucleoside kinase | - |
Methanocaldococcus jannaschii |