Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Sulfolobus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000027 | - |
geranylgeraniol | pH 7.0, 55°C, assay with membrane fraction | Sulfolobus acidocaldarius | |
0.65 | - |
ATP | pH 7.0, 55°C, assay with membrane fraction | Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Sulfolobus acidocaldarius | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
Mg2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
Mn2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
Ni2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
Zn2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + geranylgeraniol | Sulfolobus acidocaldarius | all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation | ADP + geranylgeranyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (2E,6E)-farnesol | 7% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | ADP + (2E,6E)-farnesyl phosphate | - |
? | |
ATP + all-trans-hexaprenol | 38% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | ADP + all-trans-hexaprenyl phosphate | - |
? | |
ATP + all-trans-octaprenol | 24% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | ADP + all-trans-octaprenyl phosphate | - |
? | |
ATP + geranylgeraniol | all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation | Sulfolobus acidocaldarius | ADP + geranylgeranyl phosphate | - |
? | |
ATP + geranylgeraniol | all-trans-geranylgeraniol and ATP are the best substrates, activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes. No formation of geranylgeranyl diphosphate is observed | Sulfolobus acidocaldarius | ADP + geranylgeranyl phosphate | - |
? | |
CTP + geranylgeraniol | 63% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | CDP + geranylgeranyl phosphate | - |
? | |
GTP + geranylgeraniol | 87% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | GDP + geranylgeranyl phosphate | - |
? | |
additional information | neither geraniol nor (22E,6E,10Z,14Z,18Z,22Z,26Z,30Z,34Z,38Z)-undecaprenol are active as substrates | Sulfolobus acidocaldarius | ? | - |
? | |
UTP + geranylgeraniol | 76% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | UDP + geranylgeranyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GGOH kinase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Sulfolobus acidocaldarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | activity at 70% is about 40% of the activity at 90°C, no activity below 40°C | Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
1 h, about 15% loss of activity | Sulfolobus acidocaldarius |
95 | - |
1 h, more than 90% loss of activity | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Sulfolobus acidocaldarius |