Cloned (Comment) | Organism |
---|---|
cloning and characterization of an isoform of rat SK1 with an N-terminal extension (long-rSK1). When the long-rSK1 is transfected in COS-7 cells, SK activity is 53fold increased. Substrate specificity and dependency on divalent cations of long-rSK1 are similar to those of rSK1 | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Rattus norvegicus | |
N,N-dimethylsphingosine | competitive inhibition with D-erythro-sphingosine as substrate | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ | Rattus norvegicus | |
Mg2+ | both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ | Rattus norvegicus | |
Mn2+ | both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49800 | - |
x * 49800, calculated from sequence | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | long-rSK1 is involved in cell signaling | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q1HGK5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | expression of long-rSK1 mRNA | Rattus norvegicus | - |
heart | expression of long-rSK1 mRNA, no expression of rSK1 mRNA | Rattus norvegicus | - |
kidney | expression of long-rSK1 mRNA | Rattus norvegicus | - |
liver | expression of long-rSK1 mRNA, no expression of rSK1 mRNA | Rattus norvegicus | - |
lung | expression of long-rSK1 mRNA | Rattus norvegicus | - |
spleen | expression of long-rSK1 mRNA | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-erythro-dihydrosphingosine | the activity of longrSK1 towards dihydrosphingosine is about 3.6fold higher than that of rSK1 | Rattus norvegicus | ADP + D-erythro-dihydrosphingosine 1-phosphate | - |
? | |
ATP + D-erythro-sphingosine | - |
Rattus norvegicus | ADP + D-erythro-sphingosine 1-phosphate | - |
? | |
additional information | long-rSK1 is involved in cell signaling | Rattus norvegicus | ? | - |
? | |
additional information | no phosphorylation of with threo-dihydrosphingosine, N,N-dimethylsphingosine and N-acetyl-D-sphingosine | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49800, calculated from sequence | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
SK1 | - |
Rattus norvegicus |
sphingosine kinase 1 | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
N,N-dimethylsphingosine | rSK1 | Rattus norvegicus | |
0.0058 | - |
N,N-dimethylsphingosine | long-rSK1 | Rattus norvegicus |