Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | Ca2+-dependent binding of recombinant wild-type and mutant enzymes | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged isozyme SK1 splicing variant a in HEK293 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D175N | site-directed mutagenesis, about 50% of wild-type enzyme activity | Mus musculus |
D175N/D177N | site-directed mutagenesis, less than 5% of wild-type enzyme activity | Mus musculus |
D177N | site-directed mutagenesis, less than 10% of wild-type enzyme activity | Mus musculus |
D177N/E179Q | site-directed mutagenesis, nearly inactive mutant | Mus musculus |
E179Q | site-directed mutagenesis, about 30% of wild-type enzyme activity | Mus musculus |
E181Q | site-directed mutagenesis, about 30% of wild-type enzyme activity | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant wild-type and mutant enzymes | Mus musculus | |
0.01 | - |
sphingosine | pH 7.4, 37°C, recombinant wild-type enzyme and mutant E181Q | Mus musculus | |
0.026 | - |
sphingosine | pH 7.4, 37°C, recombinant mutant E179Q | Mus musculus | |
0.037 | - |
sphingosine | pH 7.4, 37°C, recombinant mutant D175N | Mus musculus | |
0.063 | - |
ATP | pH 7.4, 37°C, recombinant mutant E179Q | Mus musculus | |
0.072 | - |
ATP | pH 7.4, 37°C, recombinant wild-type enzyme | Mus musculus | |
0.077 | - |
ATP | pH 7.4, 37°C, recombinant mutant D175N | Mus musculus | |
0.088 | - |
ATP | pH 7.4, 37°C, recombinant mutant D177N | Mus musculus | |
0.095 | - |
ATP | pH 7.4, 37°C, recombinant mutant E181Q | Mus musculus | |
0.108 | - |
sphingosine | pH 7.4, 37°C, recombinant mutant D177N | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+-dependent calmodulin binding of recombinant wild-type and mutant enzymes | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sphingosine | Mus musculus | involved in diverse biological functions | ADP + sphingosine 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8CI15 | isozyme SK1 splicing variant a | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a sphingoid base = ADP + a sphingoid base 1-phosphate | Asp177 in C4 domain of isozyme SK1a is important for sphingosine substrate recognition | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax of recombinant wild-type and mutant enzymes | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sphingosine | - |
Mus musculus | ADP + sphingosine 1-phosphate | - |
? | |
ATP + sphingosine | involved in diverse biological functions | Mus musculus | ADP + sphingosine 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SK | - |
Mus musculus |
sphinganine kinase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |