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Literature summary for 2.7.1.91 extracted from
- Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes (2000), Biochem. J., 350, 429-441.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Homo sapiens |  |
| dihydrosphingosine | - |
Homo sapiens | |
| DL-threo-dihydrosphingosine | - |
Homo sapiens | |
| EDTA | - |
Homo sapiens | |
| N,N,N-trimethylsphingosine | - |
Homo sapiens | |
| N,N-dimethylsphingosine | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
D-erythrosphinganine | as bovine serum albumin complex | Homo sapiens | |
| 0.02 | - |
DL-erythro-dihydrosphingosine | - |
Homo sapiens | |
| 0.077 | - |
ATP | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | less stimulating than Mg2+, Mn2+ | Homo sapiens | |
| Mg2+ | highest activity with | Homo sapiens | |
| Mn2+ | less stimulating than Mg2+ | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9NYA1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + DL-erythro-dihydrosphingosine | - |
Homo sapiens | ADP + DL-erythro-dihydrosphingosine 1-phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
10% glycerol, 0.05% Triton X-100, 0.5 M NaCl, stable for 30 min | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.4 | greater than 60% of maximum activity | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
D,L-threo-dihydrosphingosine | - |
Homo sapiens | |
| 0.008 | - |
N,N-dimethylsphingosine | - |
Homo sapiens | |
| 1 | - |
ADP | - |
Homo sapiens | |
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