Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Acetivibrio thermocellus |
overexpression in Escherichia coli | Pseudoclostridium thermosuccinogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
D-fructose 6-phosphate | pH 7.0, 55°C | Acetivibrio thermocellus | |
0.07 | - |
D-fructose 6-phosphate | pH 7.0, 55°C | Pseudoclostridium thermosuccinogenes | |
0.1 | - |
sedoheptulose 7-phosphate | pH 7.0, 55°C | Acetivibrio thermocellus | |
0.11 | - |
sedoheptulose 7-phosphate | pH 7.0, 55°C | Pseudoclostridium thermosuccinogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + sedoheptulose 7-phosphate | Acetivibrio thermocellus | the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase | phosphate + sedoheptulose 1,7-bisphosphate | - |
? | |
diphosphate + sedoheptulose 7-phosphate | Pseudoclostridium thermosuccinogenes | the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase | phosphate + sedoheptulose 1,7-bisphosphate | - |
? | |
diphosphate + sedoheptulose 7-phosphate | Acetivibrio thermocellus DSM 1313 | the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase | phosphate + sedoheptulose 1,7-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A0A2K2FKR3 | - |
- |
Acetivibrio thermocellus DSM 1313 | A0A2K2FKR3 | - |
- |
Pseudoclostridium thermosuccinogenes | PNT94038.1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetivibrio thermocellus |
- |
Pseudoclostridium thermosuccinogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate | Acetivibrio thermocellus | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + D-fructose 6-phosphate | the enzyme has similar affinity for D-fructose 6-phosphate sedoheptulose 7-phosphate | Pseudoclostridium thermosuccinogenes | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + D-fructose 6-phosphate | the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate | Acetivibrio thermocellus DSM 1313 | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + sedoheptulose 7-phosphate | the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase | Acetivibrio thermocellus | phosphate + sedoheptulose 1,7-bisphosphate | - |
? | |
diphosphate + sedoheptulose 7-phosphate | the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase | Pseudoclostridium thermosuccinogenes | phosphate + sedoheptulose 1,7-bisphosphate | - |
? | |
diphosphate + sedoheptulose 7-phosphate | the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate | Acetivibrio thermocellus | phosphate + sedoheptulose 1,7-bisphosphate | - |
? | |
diphosphate + sedoheptulose 7-phosphate | the enzyme has similar affinity for D-fructose 6-phosphate sedoheptulose 7-phosphate | Pseudoclostridium thermosuccinogenes | phosphate + sedoheptulose 1,7-bisphosphate | - |
? | |
diphosphate + sedoheptulose 7-phosphate | the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase | Acetivibrio thermocellus DSM 1313 | phosphate + sedoheptulose 1,7-bisphosphate | - |
? | |
diphosphate + sedoheptulose 7-phosphate | the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate | Acetivibrio thermocellus DSM 1313 | phosphate + sedoheptulose 1,7-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPi-PFK | - |
Acetivibrio thermocellus |
PPi-PFK | - |
Pseudoclostridium thermosuccinogenes |
pyrophosphate-dependent phosphofructokinase | - |
Acetivibrio thermocellus |
pyrophosphate-dependent phosphofructokinase | - |
Pseudoclostridium thermosuccinogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49 | - |
sedoheptulose 7-phosphate | pH 7.0, 55°C | Pseudoclostridium thermosuccinogenes | |
80 | - |
sedoheptulose 7-phosphate | pH 7.0, 55°C | Acetivibrio thermocellus | |
127 | - |
D-fructose 6-phosphate | pH 7.0, 55°C | Acetivibrio thermocellus | |
182 | - |
D-fructose 6-phosphate | pH 7.0, 55°C | Pseudoclostridium thermosuccinogenes |
General Information | Comment | Organism |
---|---|---|
metabolism | the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase | Acetivibrio thermocellus |
metabolism | the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase | Pseudoclostridium thermosuccinogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
460 | - |
sedoheptulose 7-phosphate | pH 7.0, 55°C | Pseudoclostridium thermosuccinogenes | |
800 | - |
sedoheptulose 7-phosphate | pH 7.0, 55°C | Acetivibrio thermocellus | |
2600 | - |
D-fructose 6-phosphate | pH 7.0, 55°C | Pseudoclostridium thermosuccinogenes | |
2800 | - |
D-fructose 6-phosphate | pH 7.0, 55°C | Acetivibrio thermocellus |