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Literature summary for 2.7.1.90 extracted from

  • Koendjbiharie, J.G.; Hon, S.; Pabst, M.; Hooftman, R.; Stevenson, D.M.; Cui, J.; Amador-Noguez, D.; Lynd, L.R.; Olson, D.G.; van Kranenburg, R.
    The pentose phosphate pathway of cellulolytic clostridia relies on 6-phosphofructokinase instead of transaldolase (2020), J. Biol. Chem., 295, 1867-1878 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Acetivibrio thermocellus
overexpression in Escherichia coli Pseudoclostridium thermosuccinogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
D-fructose 6-phosphate pH 7.0, 55°C Acetivibrio thermocellus
0.07
-
D-fructose 6-phosphate pH 7.0, 55°C Pseudoclostridium thermosuccinogenes
0.1
-
sedoheptulose 7-phosphate pH 7.0, 55°C Acetivibrio thermocellus
0.11
-
sedoheptulose 7-phosphate pH 7.0, 55°C Pseudoclostridium thermosuccinogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + sedoheptulose 7-phosphate Acetivibrio thermocellus the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase phosphate + sedoheptulose 1,7-bisphosphate
-
?
diphosphate + sedoheptulose 7-phosphate Pseudoclostridium thermosuccinogenes the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase phosphate + sedoheptulose 1,7-bisphosphate
-
?
diphosphate + sedoheptulose 7-phosphate Acetivibrio thermocellus DSM 1313 the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase phosphate + sedoheptulose 1,7-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A0A2K2FKR3
-
-
Acetivibrio thermocellus DSM 1313 A0A2K2FKR3
-
-
Pseudoclostridium thermosuccinogenes PNT94038.1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetivibrio thermocellus
-
Pseudoclostridium thermosuccinogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate Acetivibrio thermocellus phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + D-fructose 6-phosphate the enzyme has similar affinity for D-fructose 6-phosphate sedoheptulose 7-phosphate Pseudoclostridium thermosuccinogenes phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + D-fructose 6-phosphate the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate Acetivibrio thermocellus DSM 1313 phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + sedoheptulose 7-phosphate the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Acetivibrio thermocellus phosphate + sedoheptulose 1,7-bisphosphate
-
?
diphosphate + sedoheptulose 7-phosphate the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Pseudoclostridium thermosuccinogenes phosphate + sedoheptulose 1,7-bisphosphate
-
?
diphosphate + sedoheptulose 7-phosphate the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate Acetivibrio thermocellus phosphate + sedoheptulose 1,7-bisphosphate
-
?
diphosphate + sedoheptulose 7-phosphate the enzyme has similar affinity for D-fructose 6-phosphate sedoheptulose 7-phosphate Pseudoclostridium thermosuccinogenes phosphate + sedoheptulose 1,7-bisphosphate
-
?
diphosphate + sedoheptulose 7-phosphate the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Acetivibrio thermocellus DSM 1313 phosphate + sedoheptulose 1,7-bisphosphate
-
?
diphosphate + sedoheptulose 7-phosphate the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate Acetivibrio thermocellus DSM 1313 phosphate + sedoheptulose 1,7-bisphosphate
-
?

Synonyms

Synonyms Comment Organism
PPi-PFK
-
Acetivibrio thermocellus
PPi-PFK
-
Pseudoclostridium thermosuccinogenes
pyrophosphate-dependent phosphofructokinase
-
Acetivibrio thermocellus
pyrophosphate-dependent phosphofructokinase
-
Pseudoclostridium thermosuccinogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
49
-
sedoheptulose 7-phosphate pH 7.0, 55°C Pseudoclostridium thermosuccinogenes
80
-
sedoheptulose 7-phosphate pH 7.0, 55°C Acetivibrio thermocellus
127
-
D-fructose 6-phosphate pH 7.0, 55°C Acetivibrio thermocellus
182
-
D-fructose 6-phosphate pH 7.0, 55°C Pseudoclostridium thermosuccinogenes

General Information

General Information Comment Organism
metabolism the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Acetivibrio thermocellus
metabolism the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Pseudoclostridium thermosuccinogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
460
-
sedoheptulose 7-phosphate pH 7.0, 55°C Pseudoclostridium thermosuccinogenes
800
-
sedoheptulose 7-phosphate pH 7.0, 55°C Acetivibrio thermocellus
2600
-
D-fructose 6-phosphate pH 7.0, 55°C Pseudoclostridium thermosuccinogenes
2800
-
D-fructose 6-phosphate pH 7.0, 55°C Acetivibrio thermocellus