Literature summary for 2.7.1.90 extracted from

Rozova, O.N.; Khmelenina, V.N.; Vuilleumier, S.; Trotsenko, Y.A.
Characterization of recombinant pyrophosphate-dependent 6-phosphofructokinase from halotolerant methanotroph Methylomicrobium alcaliphilum 20Z (2010), Res. Microbiol., 161, 861-868.

Search for more literature for 2.7.1.90

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) cells	Methylotuvimicrobium alcaliphilum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not affected by pyruvate, citrate, serine, phosphoglycerate, phosphoenolpyruvate, ATP, ADP, AMP, NADP+, NAD+ and NADPH	Methylotuvimicrobium alcaliphilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.095	-	D-fructose 1,6-bisphosphate	in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C	Methylotuvimicrobium alcaliphilum
0.118	-	diphosphate	in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C	Methylotuvimicrobium alcaliphilum
0.64	-	D-fructose 6-phosphate	in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C	Methylotuvimicrobium alcaliphilum
1.01	-	D-sedoheptulose 6-phosphate	in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C	Methylotuvimicrobium alcaliphilum
3.4	-	phosphate	in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C	Methylotuvimicrobium alcaliphilum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	at low concentration (0.01 mM), Co2+ ions can replace Mg2+ (about 39% of the Mg2+ activity)	Methylotuvimicrobium alcaliphilum
Mg2+	required for activity	Methylotuvimicrobium alcaliphilum
Mn2+	at low concentration (0.01 mM), Mn2+ ions can replace Mg2+ (about 39% of the Mg2+ activity)	Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44500	-	4 * 44500, calculated from amino acid sequence	Methylotuvimicrobium alcaliphilum
45000	-	4 * 45000, SDS-PAGE	Methylotuvimicrobium alcaliphilum
180000	-	gel filtration	Methylotuvimicrobium alcaliphilum

Organism

Organism	UniProt	Comment	Textmining
Methylotuvimicrobium alcaliphilum	-	-	-
Methylotuvimicrobium alcaliphilum 20Z	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2-NTA column chromatography	Methylotuvimicrobium alcaliphilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + D-fructose 6-phosphate	the enzyme is highly active, nonallosteric and stringently specific to diphosphate as the phosphoryl donor	Methylotuvimicrobium alcaliphilum	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	the enzyme is highly active, nonallosteric and stringently specific to diphosphate as the phosphoryl donor	Methylotuvimicrobium alcaliphilum 20Z	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-sedoheptulose 6-phosphate	low efficiency	Methylotuvimicrobium alcaliphilum	phosphate + D-sedoheptulose 1,6-bisphosphate	-	?
diphosphate + D-sedoheptulose 6-phosphate	low efficiency	Methylotuvimicrobium alcaliphilum 20Z	phosphate + D-sedoheptulose 1,6-bisphosphate	-	?
phosphate + D-fructose 1,6-bisphosphate	the enzyme is more specific for the reverse reaction substrate D-fructose-1,6-bisphosphate than for the forward reaction substrate	Methylotuvimicrobium alcaliphilum	diphosphate + D-fructose 6-phosphate	-	r
phosphate + D-fructose 1,6-bisphosphate	the enzyme is more specific for the reverse reaction substrate D-fructose-1,6-bisphosphate than for the forward reaction substrate	Methylotuvimicrobium alcaliphilum 20Z	diphosphate + D-fructose 6-phosphate	-	r

Subunits

Subunits	Comment	Organism
homotetramer	4 * 45000, SDS-PAGE	Methylotuvimicrobium alcaliphilum
homotetramer	4 * 44500, calculated from amino acid sequence	Methylotuvimicrobium alcaliphilum

Synonyms

Synonyms	Comment	Organism
PPi-PFK	-	Methylotuvimicrobium alcaliphilum
pyrophosphate-dependent 6-phosphofructokinase	-	Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Methylotuvimicrobium alcaliphilum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Methylotuvimicrobium alcaliphilum

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Release 2023.1 (January 2023)