Literature summary for 2.7.1.90 extracted from

Reshetnikov, A.S.; Rozova, O.N.; Khmelenina, V.N.; Mustakhimov, I.I.; Beschastny, A.P.; Murrell, J.C.; Trotsenko, Y.A.
Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Methylococcus capsulatus Bath (2008), FEMS Microbiol. Lett., 288, 202-210.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Methylococcus capsulatus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	addition of citrate, pyruvate, phosphoenolpyruvate, L-serine, phosphoglycerate, ATP, ADP, AMP, NADP, NADs and NADPH to the assay mixture has no effect on activity	Methylococcus capsulatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.027	-	sedoheptulose 7-phosphate	in 0.1 M HEPES-HCl, pH 7.0, at 30°C	Methylococcus capsulatus
0.027	-	diphosphate	using D-fructose 6-phosphate as cosubstrate, in 0.1 M HEPES-HCl, pH 7.0, at 30°C	Methylococcus capsulatus
0.328	-	D-fructose 1,6-bisphosphate	in 0.1 M HEPES-HCl, pH 7.0, at 30°C	Methylococcus capsulatus
2.27	-	D-fructose 6-phosphate	in 0.1 M HEPES-HCl, pH 7.0, at 30°C	Methylococcus capsulatus
8.69	-	phosphate	using D-fructose 1,6-bisphosphate as cosubstrate, in 0.1 M HEPES-HCl, pH 7.0, at 30°C	Methylococcus capsulatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for maximal activity, Co2+ or Mn2+ can substitute for Mg2+ with 63% and 48% of maximal activity	Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	2 * 45000, SDS-PAGE	Methylococcus capsulatus
90000	-	gel filtration	Methylococcus capsulatus

Organism

Organism	UniProt	Comment	Textmining
Methylococcus capsulatus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + D-fructose 6-phosphate	strong specificity for diphosphate as a phosphoryl donor	Methylococcus capsulatus	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-ribulose 5-phosphate	-	Methylococcus capsulatus	phosphate + ?	-	?
diphosphate + sedoheptulose 7-phosphate	-	Methylococcus capsulatus	phosphate + ?	-	?
additional information	ATP, ADP, AMP, CTP, CDP, CMP, UTP, UDP, UMP, GTP, GDP, and GMP at 1 mM as well as polyphosphates (n=3, n=9, n=15 and n=45) at 0.1 mg/ml cannot substitute for diphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose 1-phosphate, D-mannose 6-phosphate, D-ribose 5-phosphate, D-ribose 1-phosphate, or D-erythrose 4-phosphate cannot substitute for D-fructose 6-phosphate as a phosphate acceptor	Methylococcus capsulatus	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 45000, SDS-PAGE	Methylococcus capsulatus

Synonyms

Synonyms	Comment	Organism
PPi-PFK	-	Methylococcus capsulatus
pyrophosphate-dependent 6-phosphofructokinase	-	Methylococcus capsulatus
pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase	-	Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Methylococcus capsulatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Methylococcus capsulatus

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Release 2023.1 (January 2023)