Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | pH-dependent activation | Ananas comosus | |
D-fructose 2,6-bisphosphate | only weak activation in the reverse reaction | Ananas comosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Ananas comosus | |
0.011 | - |
diphosphate | pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate | Ananas comosus | |
0.094 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate | Ananas comosus | |
0.15 | - |
phosphate | pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate | Ananas comosus | |
0.63 | - |
Mg2+ | pH 7.7, 25°C | Ananas comosus | |
0.89 | - |
D-fructose 6-phosphate | pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate | Ananas comosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Ananas comosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61500 | - |
2 * 61500, SDS-PAGE | Ananas comosus |
97000 | - |
gel filtration | Ananas comosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | Ananas comosus | regulatory role in glucose metabolism | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ananas comosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
40fold, to homogeneity | Ananas comosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Ananas comosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.9 | - |
reverse reaction, purified enzyme, pH 7.7, in presence of fructose 2,6-bisphosphate | Ananas comosus |
43.8 | - |
forward reaction, purified enzyme, pH 7.7, in presence of fructose 2,6-bisphosphate | Ananas comosus |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme, 25 mM Tris-HCl, pH 7.0, 20% glycerol v/v, 2 mM 2-mercaptoethanol, stable for at least 6 months | Ananas comosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | substrate Mg-diphosphate | Ananas comosus | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Ananas comosus | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | regulatory role in glucose metabolism | Ananas comosus | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 61500, SDS-PAGE | Ananas comosus |
More | dimer-monomer mixture might exist | Ananas comosus |
Synonyms | Comment | Organism |
---|---|---|
PFP | - |
Ananas comosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature-independent | Ananas comosus |
25 | - |
assay at | Ananas comosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.7 | reverse reaction, pH-independent, broad optimum | Ananas comosus |
7.7 | 7.9 | forward reaction, strongly pH-dependent | Ananas comosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ananas comosus | - |
- |
6.25 |