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Literature summary for 2.7.1.90 extracted from

  • Tripodi, K.E.J.; Podesta, F.E.
    Purification and structural and kinetic characterization of the pyrophosphate:fructose-6-phosphate 1-phosphotransferase from the Crassulacean acid metabolism plant, pineapple (1997), Plant Physiol., 113, 779-786.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate pH-dependent activation Ananas comosus
D-fructose 2,6-bisphosphate only weak activation in the reverse reaction Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Ananas comosus
0.011
-
diphosphate pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate Ananas comosus
0.094
-
D-fructose 1,6-bisphosphate pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate Ananas comosus
0.15
-
phosphate pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate Ananas comosus
0.63
-
Mg2+ pH 7.7, 25°C Ananas comosus
0.89
-
D-fructose 6-phosphate pH 7.7, 25°C, in presence of fructose 2,6-bisphosphate Ananas comosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61500
-
2 * 61500, SDS-PAGE Ananas comosus
97000
-
gel filtration Ananas comosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Ananas comosus regulatory role in glucose metabolism phosphate + D-fructose 1,6-bisphosphate
-
r

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
40fold, to homogeneity Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Ananas comosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.9
-
reverse reaction, purified enzyme, pH 7.7, in presence of fructose 2,6-bisphosphate Ananas comosus
43.8
-
forward reaction, purified enzyme, pH 7.7, in presence of fructose 2,6-bisphosphate Ananas comosus

Storage Stability

Storage Stability Organism
-80°C, purified enzyme, 25 mM Tris-HCl, pH 7.0, 20% glycerol v/v, 2 mM 2-mercaptoethanol, stable for at least 6 months Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate substrate Mg-diphosphate Ananas comosus phosphate + D-fructose 1,6-bisphosphate
-
r
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Ananas comosus phosphate + D-fructose 1,6-bisphosphate
-
r
diphosphate + D-fructose 6-phosphate regulatory role in glucose metabolism Ananas comosus phosphate + D-fructose 1,6-bisphosphate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 61500, SDS-PAGE Ananas comosus
More dimer-monomer mixture might exist Ananas comosus

Synonyms

Synonyms Comment Organism
PFP
-
Ananas comosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
temperature-independent Ananas comosus
25
-
assay at Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 8.7 reverse reaction, pH-independent, broad optimum Ananas comosus
7.7 7.9 forward reaction, strongly pH-dependent Ananas comosus

pI Value

Organism Comment pI Value Maximum pI Value
Ananas comosus
-
-
6.25