Literature summary for 2.7.1.90 extracted from

Theodorou, M.E.; Plaxton, W.C.
Purification and characterization of pyrophosphate-dependent phosphofructokinase from phosphate-starved Brassica nigra suspension cells (1996), Plant Physiol., 112, 343-351.

Search for more literature for 2.7.1.90

Activating Compound

Activating Compound	Comment	Organism	Structure
D-fructose 2,6-bisphosphate	60fold increase in affinity for fructose 6-phosphate and 3-5fold for fructose 1,6-bisohosphate	Brassica nigra
D-fructose 2,6-bisphosphate	shifts pH-optimum for both reaction directions to slightly acidic	Brassica nigra

General Stability

General Stability	Organism
chymostatin and leupeptin stabilize the enzyme and prevent degradation during purification	Brassica nigra

Inhibitors

Inhibitors	Comment	Organism	Structure
3-phospho-D-glycerate	no inhibition	Brassica nigra
ADP	forward reaction, as Mg2+-complex	Brassica nigra
AMP	no inhibition	Brassica nigra
ATP	forward reaction, as Mg2+-complex	Brassica nigra
citrate	no inhibition	Brassica nigra
D-glucose 6-phosphate	no inhibition	Brassica nigra
diphosphate	reverse reaction	Brassica nigra
additional information	no inhibition by 2-phosphoglycerate	Brassica nigra
phosphate	forward reaction, product inhibition, kinetics	Brassica nigra
phosphoenolpyruvate	forward reaction	Brassica nigra

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	0.017	diphosphate	pH 6.8, 25°C, in presence of fructose 2,6-bisphosphate	Brassica nigra
0.05	-	D-fructose 6-phosphate	pH 6.8, 25°C, in presence of fructose 2,6-bisphosphate	Brassica nigra
0.09	-	D-fructose 1,6-bisphosphate	pH 6.8, 25°C, in presence of fructose 2,6-bisphosphate	Brassica nigra
0.25	-	phosphate	pH 6.8, 25°C, in presence of fructose 2,6-bisphosphate	Brassica nigra

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	requirement	Brassica nigra

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	4 * 60000 + 4 * 66000, SDS-PAGE	Brassica nigra
66000	-	4 * 60000 + 4 * 66000, SDS-PAGE	Brassica nigra
460000	-	gel filtration	Brassica nigra
520000	-	native PAGE	Brassica nigra

Organism

Organism	UniProt	Comment	Textmining
Brassica nigra	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
184fold	Brassica nigra

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	-	Brassica nigra	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
26	-	purified enzyme	Brassica nigra

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + D-fructose 6-phosphate	specific for diphosphate as phosphor donor	Brassica nigra	phosphate + D-fructose 1,6-bisphosphate	-	r

Subunits

Subunits	Comment	Organism
octamer	4 * 60000 + 4 * 66000, SDS-PAGE	Brassica nigra

Synonyms

Synonyms	Comment	Organism
PFP	-	Brassica nigra

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Brassica nigra

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	forward reaction in presence of fructose 2,6-bisphosphate	Brassica nigra
7.6	-	reverse reaction, broad optimum, in both presence and absence of fructose 2,6-bisphosphate	Brassica nigra
7.6	-	forward reaction in absence of fructose 2,6-bisphosphate	Brassica nigra

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.8	7.6	reverse reaction, 96% activity at pH 6.8, maximum at pH 7.6	Brassica nigra

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Release 2023.1 (January 2023)