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Literature summary for 2.7.1.90 extracted from
- Pyrophosphate-dependent phosphofructokinase from Giardia lamblia: purification and characterization (1995), Protein Expr. Purif., 6, 319-328.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | no activation | Giardia intestinalis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | competitive versus D-fructose 1,6-bisphosphate, reverse reaction | Giardia intestinalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.039 | - |
diphosphate | pH 7.4, 30°C | Giardia intestinalis | |
| 0.25 | - |
D-fructose 6-phosphate | pH 7.4, 30°C | Giardia intestinalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Giardia intestinalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
1 * 64000, SDS-PAGE | Giardia intestinalis |
| 64000 | 67000 | native PAGE, gel filtration | Giardia intestinalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Giardia intestinalis | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | Giardia intestinalis Portland-1 / ATCC 30888 | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Giardia intestinalis | - |
- |
- |
| Giardia intestinalis Portland-1 / ATCC 30888 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 different methods, method 1: 292fold to homogeneity | Giardia intestinalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Giardia intestinalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 146 | - |
purified enzyme | Giardia intestinalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Giardia intestinalis | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Giardia intestinalis | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | - |
Giardia intestinalis Portland-1 / ATCC 30888 | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Giardia intestinalis Portland-1 / ATCC 30888 | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64000, SDS-PAGE | Giardia intestinalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPi-PFK | - |
Giardia intestinalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Giardia intestinalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reverse reaction | Giardia intestinalis |
| 7.2 | - |
forward reaction | Giardia intestinalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8.5 | half-maximal activity at pH 6.3 and pH 8.5 | Giardia intestinalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | 8 | 1 h, 30°C, stable | Giardia intestinalis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Giardia intestinalis | - |
- |
8.7 |
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