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Literature summary for 2.7.1.90 extracted from

  • Enomoto, T.; Ohyama, H.; Kodama, M.
    Purification and characterization of pyrophosphate: D-D-fructose 6-phosphate 1-phosphotransferase from rice seedlings (1992), Biosci. Biotechnol. Biochem., 56, 251-255.
No PubMed abstract available
Search for more literature for 2.7.1.90

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate shifts pH-optimum for both reaction directions to slightly acidic Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
phosphate in the forward reaction only in presence of fructose 2,6-bisphosphate Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters, in the absence or presence of D-fructose 2,6-bisphosphate Oryza sativa
0.139 0.21 D-fructose 1,6-bisphosphate
-
 Oryza sativa
0.59 1 phosphate
-
 Oryza sativa
3.8 10.6 D-fructose 6-phosphate with diphosphate Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 1 * 100000, SDS-PAGE Oryza sativa
103000
-
 gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Oryza sativa
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 Japonica cv. Koshihikari
-

Purification (Commentary)

Purification (Comment) Organism
852fold, to apparent homogeneity Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.6
-
 purified enzyme Oryza sativa

Storage Stability

Storage Stability Organism
4°C, 10% loss of activity within 2 weeks Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
 Oryza sativa phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Oryza sativa phosphate + D-fructose 1,6-bisphosphate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 in the presence of D-fructose 2,6-bisphosphate Oryza sativa
7.5
-
 in the presence of D-fructose 2,6-bisphosphate Oryza sativa
7.9
-
 reverse reaction, in absence of D-fructose 2,6-bisphosphate Oryza sativa
8.1
-
 forward reaction, in absence of D-fructose 2,6-bisphosphate Oryza sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.46
-
 phosphate versus fructose 6-phosphate, pH 7.5, 25°C, in presence of fructose 2,6-bisphosphate at saturating concentration Oryza sativa
1.53
-
 phosphate versus diphosphate, pH 7.5, 25°C, in presence of fructose 2,6-bisphosphate at saturating concentration Oryza sativa