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Literature summary for 2.7.1.90 extracted from
- Characterization of pyrophosphate dependent phosphofructokinase from germinating bean seeds (1987), Plant Sci., 51, 151-157.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | kinetics | Phaseolus vulgaris |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 6-phosphate | - |
Phaseolus vulgaris | |
| diphosphate | - |
Phaseolus vulgaris | |
| phosphate | - |
Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Phaseolus vulgaris | |
| 0.1 | - |
Mg2+ | pH 7.5, 25°C | Phaseolus vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Phaseolus vulgaris | |
| divalent cations | required | Phaseolus vulgaris | |
| divalent cations | desecending order Mn2+, Mg2+, Co2+ | Phaseolus vulgaris | |
| divalent cations | descending order Mg2+, Mn2+ and Co2+ | Phaseolus vulgaris | |
| Mg2+ | requirement | Phaseolus vulgaris | |
| Mn2+ | requirement | Phaseolus vulgaris | |
| additional information | no activation by Cu2+ | Phaseolus vulgaris | |
| additional information | no activation by Zn2+ | Phaseolus vulgaris | |
| additional information | no activation by Fe2+ | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Phaseolus vulgaris | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
cv. Top Crop | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Phaseolus vulgaris |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate | mechanism | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating | Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
partially purified enzyme | Phaseolus vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 10 mM HEPES-NaOH buffer, pH 7.5, 10 mM 2-mercaptoethanol, 30% v/v glycerol, several months | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Phaseolus vulgaris | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Phaseolus vulgaris |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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