Any feedback?
Literature summary for 2.7.1.90 extracted from
- Kinetic properties of pyrophosphate:D-fructose-6-phosphate phosphotransferase from germinating castor bean endosperm (1984), Plant Physiol., 74, 395-401.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | 20fold activation of forward reaction, 10fold increase in affinity for the substrates of both reaction directions | Ricinus communis |  |
General Stability
| General Stability | Organism |
|---|---|
| glycerol essential for stability during purification | Ricinus communis |
| Mg2+ stabilizes | Ricinus communis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Ricinus communis | |
| Cl- | at high concentrations | Ricinus communis | |
| D-fructose 1,6-bisphosphate | - |
Ricinus communis | |
| D-fructose 6-phosphate | - |
Ricinus communis | |
| diphosphate | - |
Ricinus communis | |
| Mg2+ | at high concentrations | Ricinus communis | |
| NH4+ | weak | Ricinus communis | |
| Ni2+ | - |
Ricinus communis | |
| NO3- | at high concentrations | Ricinus communis | |
| phosphate | inhibitory in the reverse reaction; in the forward reaction only in presence of fructose 2,6-bisphosphate | Ricinus communis | |
| SO42- | at high concentrations | Ricinus communis | |
| sulfate | weak | Ricinus communis | |
| Zn2+ | - |
Ricinus communis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | 0.017 | diphosphate | pH 7.5, 25°C | Ricinus communis | |
| 0.023 | - |
fructose 1,6-bisphosphate | reverse reaction, pH 7.8, 25°C | Ricinus communis | |
| 0.3 | - |
D-fructose 6-phosphate | forward reaction, pH 7.5, 25°C | Ricinus communis | |
| 0.63 | - |
phosphate | pH 7.8, 25°C | Ricinus communis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | less effective than Mg2+ | Ricinus communis | |
| divalent cations | required | Ricinus communis | |
| divalent cations | Mn2+ or Mg2+ | Ricinus communis | |
| Mg2+ | requirement | Ricinus communis | |
| Mg2+ | inhibitory at high concentration | Ricinus communis | |
| Mn2+ | requirement | Ricinus communis | |
| Mn2+ | less effective than Mg2+ | Ricinus communis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Ricinus communis | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
var. Hale | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 573fold | Ricinus communis |
| partial | Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Ricinus communis | - |
| seed | germinating | Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.25 | - |
purified enzyme | Ricinus communis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in glycerol, 20% w/v, 20% loss of activity within 1 week | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Ricinus communis | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Ricinus communis | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.7 | forward reaction | Ricinus communis |
| 7.8 | - |
reverse reaction | Ricinus communis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
diphosphate | forward reaction, pH 7.5, 25°C | Ricinus communis | |
| 0.013 | - |
D-fructose 1,6-bisphosphate | reverse reaction, pH 7.8, 25°C | Ricinus communis | |
| 0.113 | - |
D-fructose 6-phosphate | forward reaction, pH 7.5, 25°C | Ricinus communis | |
| 0.39 | - |
phosphate | reverse reaction, pH 7.8, 25°C | Ricinus communis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
