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Literature summary for 2.7.1.90 extracted from
- Inhibition of pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase by imidodiphosphate (1992), Plant Sci., 86, 183-189.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 6-phosphate | reverse reaction, product inhibition, kinetics | Solanum tuberosum |  |
| D-fructose 6-phosphate | - |
Spinacia oleracea | |
| diphosphate | kinetics; reverse reaction | Solanum tuberosum | |
| diphosphate | - |
Spinacia oleracea | |
| Imidodiphosphate | mixed inhibitor with respect to both fructose 6-phosphate and diphosphate | Solanum tuberosum | |
| Imidodiphosphate | inhibition in leaves in vivo when fed | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | 0.017 | diphosphate | pH 8.0, 25°C | Solanum tuberosum | |
| 0.53 | - |
D-fructose 6-phosphate | pH 8.0, 25°C | Solanum tuberosum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Solanum tuberosum | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Spinacia oleracea | |
| Mg2+ | requirement | Solanum tuberosum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Spinacia oleracea | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | Solanum tuberosum | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
cv. Maris Piper | - |
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| approximately 700fold | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
| tuber | mature | Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.6 | - |
partially purified enzyme | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Spinacia oleracea | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | - |
Solanum tuberosum | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Spinacia oleracea | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Solanum tuberosum | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Spinacia oleracea |
| 25 | - |
assay at | Solanum tuberosum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic product inhibition | Solanum tuberosum |
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