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Literature summary for 2.7.1.90 extracted from
- Carbohydrate substrate specificity of bacterial and plant pyrophosphate-dependent phosphofructokinases (1986), Biochemistry, 25, 4674-4681.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | - |
Vigna radiata |  |
| D-fructose 2,6-bisphosphate | no activation | Propionibacterium freudenreichii subsp. shermanii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,6-hexanediol-monophosphate | - |
Propionibacterium freudenreichii subsp. shermanii | |
| 1,n-Alkanediol biphosphates | n: 2-9, weak | Propionibacterium freudenreichii subsp. shermanii | |
| 1,n-Alkanediol biphosphates | n: 2-9, weak | Vigna radiata | |
| 2,3-O-isopropylidene-alpha-L-sorbofuranose-1-phosphate | weak | Propionibacterium freudenreichii subsp. shermanii | |
| 2,3-O-isopropylidene-alpha-L-sorbofuranose-1-phosphate | weak | Vigna radiata | |
| 2,5-anhydro-D-glucitol-6-phosphate | - |
Propionibacterium freudenreichii subsp. shermanii | |
| 2,5-anhydro-D-glucitol-6-phosphate | - |
Vigna radiata | |
| D-arabinose-5-phosphate | weak | Propionibacterium freudenreichii subsp. shermanii | |
| D-arabinose-5-phosphate | weak | Vigna radiata | |
| D-fructose 2,6-bisphosphate | at high concentration; competitive versus D-fructose 1,6-bisphosphate, reverse reaction | Propionibacterium freudenreichii subsp. shermanii | |
| D-glucitol-6-phosphate | weak | Propionibacterium freudenreichii subsp. shermanii | |
| D-glucitol-6-phosphate | weak | Vigna radiata | |
| D-tagatose-6-phosphate | - |
Propionibacterium freudenreichii subsp. shermanii | |
| D-tagatose-6-phosphate | - |
Vigna radiata | |
| diethylene glycol-monophosphate | weak | Propionibacterium freudenreichii subsp. shermanii | |
| diethylene glycol-monophosphate | weak | Vigna radiata | |
| L-sorbose-6-phosphate | weak | Propionibacterium freudenreichii subsp. shermanii | |
| L-sorbose-6-phosphate | weak | Vigna radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
D-fructose 6-phosphate | pH 8.0, 25°C | Vigna radiata | |
| 0.24 | - |
D-fructose 6-phosphate | pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 0.38 | - |
sedoheptulose 7-phosphate | pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 0.49 | - |
sedoheptulose 7-phosphate | pH 8.0, 25°C | Vigna radiata | |
| 0.92 | - |
2,5-anhydro-D-mannitol-6-phosphate | pH 8.0, 25°C | Vigna radiata | |
| 0.97 | - |
2,5-anhydro-D-mannitol-6-phosphate | pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Propionibacterium freudenreichii subsp. shermanii | |
| Mg2+ | requirement | Vigna radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
gel filtration | Vigna radiata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Propionibacterium freudenreichii subsp. shermanii | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | Vigna radiata | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
commercial product | - |
| Propionibacterium freudenreichii subsp. shermanii | - |
subspecies of Propionibacterium freudenreichii | - |
| Vigna radiata | - |
mung bean | - |
| Vigna radiata | - |
commercial product | - |
| Vigna radiata | - |
i.e. Phaseolus aureus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification to homogeneity from a commercial lyophilized product | Propionibacterium freudenreichii subsp. shermanii |
| purification to homogeneity of a commercial lyophilized product | Vigna radiata |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate | rapid equilibrium random mechanism | Propionibacterium freudenreichii subsp. shermanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 74 | 82 | purified enzyme | Vigna radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + 2,5-anhydro-D-mannitol 6-phosphate | - |
Propionibacterium freudenreichii subsp. shermanii | phosphate + 2,5-anhydro-D-mannitol 1,6-bisphosphate | - |
r | |
| diphosphate + 2,5-anhydro-D-mannitol 6-phosphate | - |
Vigna radiata | phosphate + 2,5-anhydro-D-mannitol 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | - |
Propionibacterium freudenreichii subsp. shermanii | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | - |
Vigna radiata | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | favored anomeric, epimeric and tautomeric form of sugar substrate is the beta-D-fructofuranose | Propionibacterium freudenreichii subsp. shermanii | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | favored anomeric, epimeric and tautomeric form of sugar substrate is the beta-D-fructofuranose | Vigna radiata | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Propionibacterium freudenreichii subsp. shermanii | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Vigna radiata | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-sedoheptulose 7-phosphate | - |
Propionibacterium freudenreichii subsp. shermanii | phosphate + D-sedoheptulose 1,7-bisphosphate | - |
r | |
| diphosphate + sedoheptulose 7-phosphate | - |
Vigna radiata | phosphate + D-sedoheptulose 1,7-bisphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Propionibacterium freudenreichii subsp. shermanii |
| 8 | - |
assay at | Vigna radiata |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, various inhibitors, forward and reverse reaction | Propionibacterium freudenreichii subsp. shermanii | |
| additional information | - |
additional information | kinetics, various inhibitors, forward and reverse reaction | Vigna radiata | |
| 0.32 | - |
2,5-anhydro-D-glucitol-6-phosphate | forward reaction, pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 1.49 | - |
diethylene glycol-monophosphate | forward reaction, pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 1.52 | - |
2,5-anhydro-D-glucitol-6-phosphate | forward reaction, pH 8.0, 25°C | Vigna radiata | |
| 2.05 | - |
L-sorbose-6-phosphate | forward reaction, pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 2.16 | - |
D-glucitol-6-phosphate | forward reaction, pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 2.22 | - |
1,6-hexanediol-monophosphate | forward reaction, pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 2.37 | - |
2,3-O-isopropylidene-alpha-L-sorbofuranose-1-phosphate | forward reaction, pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 2.39 | - |
L-sorbose-6-phosphate | forward reaction, pH 8.0, 25°C | Vigna radiata | |
| 2.41 | - |
D-tagatose-6-phosphate | forward reaction, pH 8.0, 25°C | Vigna radiata | |
| 2.56 | - |
D-tagatose-6-phosphate | forward reaction, pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 3.62 | - |
D-arabinose-5-phosphate | forward reaction, pH 8.0, 25°C | Vigna radiata | |
| 3.9 | - |
D-fructose 2,6-bisphosphate | pH 8.0, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 5.07 | - |
D-glucitol-6-phosphate | forward reaction, pH 8.0, 25°C | Vigna radiata | |
| 6.7 | - |
2,3-O-isopropylidene-alpha-L-sorbofuranose-1-phosphate | forward reaction, pH 8.0, 25°C | Vigna radiata | |
| 10.87 | - |
diethylene glycol-monophosphate | forward reaction, pH 8.0, 25°C | Vigna radiata | |
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