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Literature summary for 2.7.1.90 extracted from
- PPi-dependent phosphofructotransferase (phosphofructokinase) activity in the mollicutes (mycoplasma) Acholeplasma laidlawii (1986), J. Bacteriol., 165, 53-60.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | no activation | Acholeplasma laidlawii |  |
| D-fructose 2,6-bisphosphate | no activation | Acholeplasma laidlawii | |
| additional information | no activation by or phosphoenolpyruvate, AMP, GDP, citrate, and ammonium sulfate | Acholeplasma laidlawii | |
| additional information | no activation by ATP | Acholeplasma laidlawii | |
| additional information | no activation by polyphosphate, nucleoside triphosphates | Acholeplasma laidlawii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | no inhibition | Acholeplasma laidlawii | |
| AMP | no inhibition | Acholeplasma laidlawii | |
| AMP | no inhibition | Mesoplasma florum | |
| citrate | no inhibition | Acholeplasma laidlawii | |
| citrate | no inhibition | Mesoplasma florum | |
| Co2+ | inhibitory at high concentration | Acholeplasma laidlawii | |
| Mn2+ | inhibitory at high concentration | Acholeplasma laidlawii | |
| additional information | no inhibition by ammonium sulfate, D-fructose 2,6-bisphosphate, GDP; no inhibition by nucleoside triphosphates | Acholeplasma laidlawii | |
| phosphoenolpyruvate | no inhibition | Acholeplasma laidlawii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Acholeplasma laidlawii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Acholeplasma laidlawii | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | less effective than Mg2+ | Acholeplasma laidlawii | |
| Co2+ | less effective than Mn2+ | Acholeplasma laidlawii | |
| Co2+ | inhibitory at high concentration | Acholeplasma laidlawii | |
| divalent cations | required | Acholeplasma laidlawii | |
| divalent cations | descending order Mg2+, Mn2+ and Co2+ | Acholeplasma laidlawii | |
| divalent cations | Mn2+ or Mg2+ | Acholeplasma laidlawii | |
| Mg2+ | requirement | Acholeplasma laidlawii | |
| Mg2+ | requirement | Mesoplasma florum | |
| Mn2+ | requirement | Acholeplasma laidlawii | |
| Mn2+ | requirement | Mesoplasma florum | |
| Mn2+ | less effective than Mg2+ | Acholeplasma laidlawii | |
| Mn2+ | inhibitory at high concentration | Acholeplasma laidlawii | |
| additional information | no activation by Zn2+ | Acholeplasma laidlawii | |
| additional information | no activation by (NH4)2SO4 | Acholeplasma laidlawii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
sucrose density gradient centrifugation | Acholeplasma laidlawii |
| 81000 | - |
PAGE | Acholeplasma laidlawii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Acholeplasma laidlawii | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | Mesoplasma florum | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | Mesoplasma florum L1 | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | Acholeplasma laidlawii B-PG9 | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acholeplasma laidlawii | - |
- |
- |
| Acholeplasma laidlawii B-PG9 | - |
- |
- |
| Mesoplasma florum | - |
- |
- |
| Mesoplasma florum L1 | - |
- |
- |
| no activity in Mycoplasma gallisepticum S6 | - |
- |
- |
| no activity in Spiroplasma floricola | - |
- |
- |
| no activity in Spiroplasma floricola 23-6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 430fold | Acholeplasma laidlawii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00016 | - |
crude enzyme extract, phosphor donor in the forward reaction is diphosphate | Mesoplasma florum |
| 0.00021 | - |
crude enzyme extract, phosphor donor in the forward reaction is ATP | Mesoplasma florum |
| 12 | - |
partially purified enzyme | Acholeplasma laidlawii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in kappa-buffer with glycerol, 10% v/v, about 25% loss of activity within 6 months | Acholeplasma laidlawii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenate + D-fructose 1,6-bisphosphate | specific for phosphate or arsenate in the reverse reaction | Acholeplasma laidlawii | ? | - |
r | |
| arsenate + D-fructose 1,6-bisphosphate | specific for phosphate or arsenate in the reverse reaction | Acholeplasma laidlawii B-PG9 | ? | - |
r | |
| diphosphate + D-fructose 6-phosphate | - |
Acholeplasma laidlawii | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | - |
Mesoplasma florum | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | phosphate cannot replace diphosphate and vice versa | Mesoplasma florum | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | phosphate cannot replace diphosphate and vice versa | Acholeplasma laidlawii | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Mesoplasma florum | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Acholeplasma laidlawii | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
| diphosphate + D-fructose 6-phosphate | - |
Mesoplasma florum L1 | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | phosphate cannot replace diphosphate and vice versa | Mesoplasma florum L1 | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Mesoplasma florum L1 | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | - |
Acholeplasma laidlawii B-PG9 | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | phosphate cannot replace diphosphate and vice versa | Acholeplasma laidlawii B-PG9 | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Acholeplasma laidlawii B-PG9 | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | 22 | assay at | Acholeplasma laidlawii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
2 min, about 60% loss of activity, inactivation within 10 min | Acholeplasma laidlawii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Acholeplasma laidlawii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 10.3 | smooth hyperbolic pH-value/activity curve | Acholeplasma laidlawii |
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