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Literature summary for 2.7.1.90 extracted from

  • Peng, Z.Y.; Mansour, T.E.
    Purification and properties of a pyrophosphate-dependent phosphofructokinase from Toxoplasma gondii (1992), Mol. Biochem. Parasitol., 54, 223-230.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate no activation Toxoplasma gondii
additional information no activation by ATP Toxoplasma gondii
additional information no activation by polyphosphate, nucleoside triphosphates Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
ATP no inhibition Toxoplasma gondii
additional information no inhibition by nucleoside triphosphates Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025 0.033 diphosphate
-
Toxoplasma gondii
0.27
-
D-fructose 6-phosphate pH 7.5, 30°C Toxoplasma gondii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
2 * 45000, SDS-PAGE Toxoplasma gondii
100000
-
gel filtration Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Toxoplasma gondii
-
phosphate + D-fructose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
Toxoplasma gondii phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Toxoplasma gondii phosphate + D-fructose 1,6-bisphosphate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Toxoplasma gondii