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Literature summary for 2.7.1.90 extracted from

  • Mertens, E.; De Jonckheere, J.; Van Schaftingen, E.
    Pyrophosphate-dependent phosphofructokinase from the amoeba Naegleria fowleri, an AMP-sensitive enzyme (1993), Biochem. J., 292, 797-803.
No PubMed abstract available
Search for more literature for 2.7.1.90

Activating Compound

Activating Compound Comment Organism Structure
adenosine slight activation Naegleria fowleri
Aminoimidazolecarboxamide riboside about 25% as efficient as AMP Naegleria fowleri
AMP activation, Triton X-100 enhances stimulation by AMP Naegleria fowleri
cAMP slight activation Naegleria fowleri
CMP slight activation Naegleria fowleri
D-fructose 2,6-bisphosphate no activation Naegleria fowleri
dAMP activation, about 25% as efficient as AMP Naegleria fowleri
GMP activation, about 35% as efficient as AMP Naegleria fowleri
IMP activation, about 35% as efficient as AMP Naegleria fowleri
MgADP- activation, about 15% as efficient as AMP Naegleria fowleri
MgATP2- activation, about 15% as efficient as AMP Naegleria fowleri
additional information no activation by ATP Naegleria fowleri
additional information no activation by ATP Naegleria gruberi
NAD+ slight activation Naegleria fowleri
phosphate slight activation Naegleria fowleri
Polyethylene glycol stimulation, synergistic with AMP Naegleria fowleri
UMP slight activation Naegleria fowleri
XMP slight activation Naegleria fowleri

General Stability

General Stability Organism
gel filtration inactivates, AMP restores activity, more rapidly at 30°C than at 0°C Naegleria fowleri

Inhibitors

Inhibitors Comment Organism Structure
ATP no inhibition Naegleria fowleri
KSCN
-
 Naegleria fowleri
additional information no inhibition by polyphosphate Naegleria fowleri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 D-fructose 6-phosphate pH 7.0, 30°C Naegleria fowleri
0.014 0.017 diphosphate
-
 Naegleria gruberi
0.014 0.017 diphosphate pH 7.0, 30°C Naegleria fowleri
0.035
-
 D-fructose 1,6-bisphosphate pH 7.0, 30°C Naegleria fowleri
0.59
-
 phosphate pH 7.0, 30°C Naegleria fowleri
0.59 1 phosphate
-
 Naegleria gruberi

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations required Naegleria fowleri
divalent cations required Naegleria gruberi
divalent cations Mn2+ or Mg2+ Naegleria fowleri
divalent cations Mn2+ or Mg2+ Naegleria gruberi
Mg2+ requirement Naegleria fowleri
Mg2+ requirement Naegleria gruberi
Mn2+ requirement Naegleria fowleri
Mn2+ requirement Naegleria gruberi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 4 * 51000, SDS-PAGE Naegleria fowleri
180000
-
 gel filtration Naegleria fowleri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Naegleria fowleri
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate Naegleria gruberi
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate Naegleria gruberi B G-6
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate Naegleria fowleri KVL-
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Naegleria fowleri
-
 KVL-strain
-
Naegleria fowleri KVL-
-
 KVL-strain
-
Naegleria gruberi
-
 B G-6-strain
-
Naegleria gruberi B G-6
-
 B G-6-strain
-
no activity in Acanthamoeba castellani
-
-
-
no activity in Acanthamoeba castellani NEFF
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1124fold to near homogeneity Naegleria fowleri
inactivation by gel filtration, reactivation by AMP Naegleria fowleri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65
-
 purified enzyme Naegleria fowleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
 Naegleria fowleri phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate
-
 Naegleria gruberi phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Naegleria fowleri phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Naegleria gruberi phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate
-
 Naegleria gruberi B G-6 phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Naegleria gruberi B G-6 phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate
-
 Naegleria fowleri KVL- phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Naegleria fowleri KVL- phosphate + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 51000, SDS-PAGE Naegleria fowleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Naegleria fowleri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 slight inactivation Naegleria fowleri
30
-
 at enzyme concentrations of more than 10-20 units/ml, partial activation, enhanced by AMP, crude cell extract: t1/2: 20 min, AMP stabilizes, 25% loss of activity within 2 h Naegleria fowleri
30
-
 at enzyme concentrations of more than 10-20 units/ml, partial activation, enhanced by AMP, crude cell extract: t1/2: 20 min, AMP stabilizes, 25% loss of activity within 2 h Naegleria gruberi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 forward reaction Naegleria fowleri
7.5
-
 reverse reaction Naegleria fowleri