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Literature summary for 2.7.1.90 extracted from

  • Turner, W.L.; Plaxton, W.C.
    Purification and characterization of pyrophosphate- and ATP-dependent phosphofructokinases from banana fruit (2003), Planta, 217, 113-121.
    View publication on PubMed
Search for more literature for 2.7.1.90

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-diphosphate up to 24fold enhancement of glycolytic enzyme activity Musa cavendishii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0097
-
 diphosphate pH 7.0, 25°C, presence of 0.005 mM D-fructose 1,6-diphosphate Musa cavendishii
0.025
-
 D-fructose 1,6-diphosphate pH 7.0, 25°C, presence of 0.005 mM D-fructose 1,6-diphosphate Musa cavendishii
0.032
-
 D-fructose 6-phosphate pH 7.0, 25°C, presence of 0.005 mM D-fructose 1,6-diphosphate Musa cavendishii
0.082
-
 D-fructose 1,6-diphosphate pH 7.0, 25°C Musa cavendishii
0.093
-
 diphosphate pH 7.0, 25°C Musa cavendishii
0.38
-
 phosphate pH 7.0, 25°C Musa cavendishii
0.41
-
 phosphate pH 7.0, 25°C, presence of 0.005 mM D-fructose 1,6-diphosphate Musa cavendishii
1.1
-
 D-fructose 6-phosphate pH 7.0, 25°C Musa cavendishii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 4 * 66000, alpha-subunit, + 4 * 60000, beta-subunit, SDS-PAGE Musa cavendishii
66000
-
 4 * 66000, alpha-subunit, + 4 * 60000, beta-subunit, SDS-PAGE Musa cavendishii
490000
-
 gel filtration Musa cavendishii

Organism

Organism UniProt Comment Textmining
Musa cavendishii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Musa cavendishii

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Musa cavendishii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.9
-
 pH 7.0, 25°C Musa cavendishii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
 Musa cavendishii phosphate + D-fructose 1,6-diphosphate
-
 r
phosphate + D-fructose 1,6-diphosphate
-
 Musa cavendishii diphosphate + D-fructose 6-phosphate
-
 r

Subunits

Subunits Comment Organism
octamer 4 * 66000, alpha-subunit, + 4 * 60000, beta-subunit, SDS-PAGE Musa cavendishii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 braod Musa cavendishii