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Literature summary for 2.7.1.90 extracted from
- Comparison of activities and properties of pyrophosphate- and adenosine triphosphate-dependent phosphofructokinases of black gram (Phaseolus mungo) seeds (1984), J. Plant Physiol., 116, 241-252.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | - |
Vigna radiata |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | no inhibition | Vigna radiata | |
| Mg2+ | at high concentrations | Vigna radiata | |
| phosphate | - |
Vigna radiata | |
| phosphoenolpyruvate | no inhibition | Vigna radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
diphosphate | - |
Vigna radiata | |
| 3.8 | 10.6 | D-fructose 6-phosphate | with diphosphate | Vigna radiata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Vigna radiata | |
| Mg2+ | inhibitory at high concentration | Vigna radiata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Vigna radiata | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
mung bean | - |
| Vigna radiata | - |
i.e. Phaseolus mungo or Vigna mungo | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Vigna radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating | Vigna radiata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Vigna radiata | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Vigna radiata | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Vigna radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Vigna radiata |
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