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Literature summary for 2.7.1.86 extracted from
- Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana (2005), Mol. Genet. Genomics, 273, 10-19.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | 0.5 mM | Arabidopsis thaliana |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Arabidopsis thaliana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Arabidopsis thaliana | |
| additional information | no significant increase in NAD(H) kinase activity is detected in the presence of Ca2+ /calmodulin | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
SDS-PAGE | Arabidopsis thaliana |
| 58250 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q56YN3 | ecotype Ws-4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap Chelating HP column chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.4 | - |
in the presence of 0.5 mM EGTA, using NAD+ as a substrate | Arabidopsis thaliana |
| 5.75 | - |
in the presence of 0.5 mM EGTA, using NADH as a substrate | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NAD+ | - |
Arabidopsis thaliana | ADP + NADP+ | - |
? | |
| ATP + NADH | shows a 2fold preference for NADH over NAD+ | Arabidopsis thaliana | ADP + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD(H)K | - |
Arabidopsis thaliana |
| NADK-1 | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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