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Literature summary for 2.7.1.85 extracted from

  • Thompson, J.; Lichtenthaler, F.W.; Peters, S.; Pikis, A.
    beta-Glucoside kinase (BglK) from Klebsiella pneumoniae. Purification, properties, and preparative synthesis of 6-phospho-beta-D-glucosides (2002), J. Biol. Chem., 277, 34310-34321.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning and high expression of His6BglK in Escherichia coli TOP10 Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
D103G catalytically inactive protein Klebsiella pneumoniae
D7G catalytically inactive protein Klebsiella pneumoniae
G131A catalytically inactive protein Klebsiella pneumoniae
G133A catalytically inactive protein Klebsiella pneumoniae
G9A catalytically inactive protein Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
NEM considerable protection Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
n-octyl beta-D-glucopyranoside pH 7.5 Klebsiella pneumoniae
0.18
-
Salicin pH 7.5 Klebsiella pneumoniae
0.21
-
arbutin pH 7.5 Klebsiella pneumoniae
0.24
-
ATP pH 7.5 Klebsiella pneumoniae
0.47
-
cellobiose pH 7.5 Klebsiella pneumoniae
0.47
-
amygdalin pH 7.5 Klebsiella pneumoniae
0.56
-
gentiobiose pH 7.5 Klebsiella pneumoniae
0.63
-
laminaribiose pH 7.5 Klebsiella pneumoniae
0.65
-
phenyl beta-D-glucopyranoside pH 7.5 Klebsiella pneumoniae
1.56
-
cellobiitol pH 7.5 Klebsiella pneumoniae
2.03
-
isopropyl beta-D-thioglucopyranoside pH 7.5 Klebsiella pneumoniae
2.23
-
methyl beta-D-glucopyranoside pH 7.5 Klebsiella pneumoniae
3.15
-
sophorose pH 7.5 Klebsiella pneumoniae
10.54
-
thiocellobiose pH 7.5 Klebsiella pneumoniae
40.29
-
D-glucose pH 7.5 Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Listeria monocytogenes
32000
-
x * 32000, SDS-PAGE Listeria innocua
32697
-
4 * 32697, calculation from nucleotide sequence Klebsiella pneumoniae
33000
-
4 * 33000, non-covalently linked subunits, SDS-PAGE Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
ATCC 23357
-
Listeria innocua
-
CLIP 11262
-
Listeria innocua CLIP 11262
-
CLIP 11262
-
Listeria monocytogenes
-
EGD-e
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
-
Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + amygdalin
-
Klebsiella pneumoniae ADP + amygdalin 6-phosphate
-
?
ATP + arbutin
-
Klebsiella pneumoniae ADP + arbutin 6-phosphate
-
?
ATP + cellobiitol
-
Klebsiella pneumoniae ?
-
?
ATP + cellobiose
-
Klebsiella pneumoniae ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
ATP + cellobiose
-
Listeria monocytogenes ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
ATP + cellobiose
-
Listeria innocua ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
ATP + cellobiose
-
Listeria innocua CLIP 11262 ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
ATP + D-glucose
-
Klebsiella pneumoniae ADP + D-glucose 6-phosphate
-
?
ATP + gentiobiose
-
Klebsiella pneumoniae ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose
-
?
ATP + isopropyl beta-D-thioglucopyranoside
-
Klebsiella pneumoniae ADP + isopropyl beta-D-thioglucopyranoside 6-phosphate
-
?
ATP + isopropyl beta-D-thioglucopyranoside
-
Klebsiella pneumoniae ?
-
?
ATP + laminaribiose
-
Klebsiella pneumoniae ADP + 6-phospho-beta-D-glucopyranosyl-(1,3)-D-glucose
-
?
ATP + methyl beta-D-glucoside
-
Klebsiella pneumoniae ADP + methyl beta-D-glucoside 6-phosphate
-
?
ATP + n-octyl-beta-D-glucopyranoside
-
Klebsiella pneumoniae ADP + n-octyl-beta-D-glucopyranoside 6-phosphate
-
?
ATP + phenyl beta-D-glucoside
-
Klebsiella pneumoniae ADP + phenyl beta-D-glucoside 6-phosphate
-
?
ATP + salicin
-
Klebsiella pneumoniae ADP + salicin 6-phosphate
-
?
ATP + sophorose
-
Klebsiella pneumoniae ADP + 6-phospho-beta-D-glucopyranosyl-(1,2)-D-glucose
-
?
ATP + thiocellobiose
-
Klebsiella pneumoniae ?
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Listeria monocytogenes
? x * 32000, SDS-PAGE Listeria innocua
tetramer 4 * 32697, calculation from nucleotide sequence Klebsiella pneumoniae
tetramer 4 * 33000, non-covalently linked subunits, SDS-PAGE Klebsiella pneumoniae